CC, Kinesin-1 CC domain; −, not observed; ±, weak and variable localization to a subset of MTs; +, weak association; ++, strong association. Classifications were determined by live cell imaging. The ± constructs were designated as variable because they weakly localized to a subset of apparently bundled MTs in some interphase cells. MT association of NOD485 was designated as weak (+) because it was not retained after fixation with paraformaldehyde and permeabilization with detergent during preparations for immunofluorescence.