| Characteristic . | Hair cell . | P0, apex of the cochlea . | P9, apex of the cochlea . | ||
|---|---|---|---|---|---|
| . | . | Control . | Myo3a−/−Myo3b−/− . | Control . | Myo3a−/−Myo3b−/− . |
| Projected height | IHC | 1.04 ± 0.28 (n = 17) | 2.04 ± 0.50 (n = 13)*** | 1.45 ± 0.38 (n = 6) | 2.33 ± 0.57 (n = 12)** |
| OHC | 0.74 ± 0.36 (n = 31) | 0.94 ± 0.31 (n = 83)** | 0.47 ± 0.17 (n = 22) | 0.95 ± 0.24 (n = 39)*** | |
| Number of stereocilia | IHC | 25.4 ± 5.4 (n = 17) | 39.9 ± 5.3 (n = 13)*** | 18.3 ± 4.95 (n = 6) | 25.5 ± 7.3 (n = 12)* |
| OHC | 31.1 ± 4.3 (n = 31) | 42.7 ± 9.2 (n = 95)*** | 30.1 ± 3.1 (n = 34) | 28.3 ± 9.96 (n = 55)ns | |
| Interstereocilia distance | IHC | 0.33 ± 0.09 (n = 17) | 0.20 ± 0.04 (n = 13)*** | 0.41 ± 0.10 (n = 6) | 0.48 ± 0.15 (n = 12)ns |
| OHC | 0.18 ± 0.02 (n = 31) | 0.18 ± 0.07 (n = 95)ns | 0.29 ± 0.04 (n = 34) | 0.32 ± 0.09 (n = 55)ns | |
| Interrow distance | IHC | 0.39 ± 0.09 (n = 17) | 0.24 ± 0.04 (n = 13)*** | 0.56 ± 0.21 (n = 6) | 0.53 ± 0.10 (n = 12)ns |
| OHC | 0.17 ± 0.03 (n = 29) | 0.21 ± 0.05 (n = 83)*** | 0.35 ± 0.07 (n = 50) | 0.32 ± 0.07 (n = 57)* | |
| Number of “side” rows | IHC | 0.0 ± 0.0 (n = 17) | 1.62 ± 0.87 (n = 13)*** | 0.0 ± 0.0 (n = 6) | 0.92 ± 0.51 (n = 12)*** |
| OHC | 0.0 ± 0.0 (n = 31) | 1.20 ± 0.85 (n = 95)*** | 0.0 ± 0.0 (n = 28) | 0.89 ± 0.88 (n = 55)*** | |
| Characteristic . | Hair cell . | P0, apex of the cochlea . | P9, apex of the cochlea . | ||
|---|---|---|---|---|---|
| . | . | Control . | Myo3a−/−Myo3b−/− . | Control . | Myo3a−/−Myo3b−/− . |
| Projected height | IHC | 1.04 ± 0.28 (n = 17) | 2.04 ± 0.50 (n = 13)*** | 1.45 ± 0.38 (n = 6) | 2.33 ± 0.57 (n = 12)** |
| OHC | 0.74 ± 0.36 (n = 31) | 0.94 ± 0.31 (n = 83)** | 0.47 ± 0.17 (n = 22) | 0.95 ± 0.24 (n = 39)*** | |
| Number of stereocilia | IHC | 25.4 ± 5.4 (n = 17) | 39.9 ± 5.3 (n = 13)*** | 18.3 ± 4.95 (n = 6) | 25.5 ± 7.3 (n = 12)* |
| OHC | 31.1 ± 4.3 (n = 31) | 42.7 ± 9.2 (n = 95)*** | 30.1 ± 3.1 (n = 34) | 28.3 ± 9.96 (n = 55)ns | |
| Interstereocilia distance | IHC | 0.33 ± 0.09 (n = 17) | 0.20 ± 0.04 (n = 13)*** | 0.41 ± 0.10 (n = 6) | 0.48 ± 0.15 (n = 12)ns |
| OHC | 0.18 ± 0.02 (n = 31) | 0.18 ± 0.07 (n = 95)ns | 0.29 ± 0.04 (n = 34) | 0.32 ± 0.09 (n = 55)ns | |
| Interrow distance | IHC | 0.39 ± 0.09 (n = 17) | 0.24 ± 0.04 (n = 13)*** | 0.56 ± 0.21 (n = 6) | 0.53 ± 0.10 (n = 12)ns |
| OHC | 0.17 ± 0.03 (n = 29) | 0.21 ± 0.05 (n = 83)*** | 0.35 ± 0.07 (n = 50) | 0.32 ± 0.07 (n = 57)* | |
| Number of “side” rows | IHC | 0.0 ± 0.0 (n = 17) | 1.62 ± 0.87 (n = 13)*** | 0.0 ± 0.0 (n = 6) | 0.92 ± 0.51 (n = 12)*** |
| OHC | 0.0 ± 0.0 (n = 31) | 1.20 ± 0.85 (n = 95)*** | 0.0 ± 0.0 (n = 28) | 0.89 ± 0.88 (n = 55)*** | |
Geometric parameters of hair bundles (mean ± SD). All distances are in micrometers. The interrow distance is defined as the mean distance between the three rows of stereocilia. All other parameters refer to the tallest row of stereocilia. Significance of the comparison of the values in Myo3a−/−Myo3b−/− and control mice according to Welsh’s t test is indicated by asterisks (*, P < 0.05; **, P < 0.01; ***, P < 0.001; ns, non-significant).