| . | Mdm12dmLys . | Nonmodified Mdm12 . | |||
|---|---|---|---|---|---|
| . | +FOS-MEA10 . | Native . | SeMet . | Full-length (Mdm12FL) . | |
| Space group | P1 | P321 | P6122 | P6122 | P6122 |
| Unit cell parameters a, b, c (Å) | 42.87, 48.52, 130.13 | 93.39, 93.39, 81.13 | 136.90, 136.90, 76.56 | 137.17, 137.17, 76.62 | 137.17, 137.17, 76.62 |
| a, b, g (°) | 90.01, 91.50, 90.01 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 |
| Wavelength (Å) | 1 | 1 | 1 | 0.979 | 1 |
| Resolution range (Å) | 50-2.25 | 50-3.31 | 50-3.10 | 50-3.40 | 50-3.50 |
| Number of observed reflections | 527,803 | 535,031 | 1,608,644 | 977,533 | 235,394 |
| Redundancya | 3.8 (3.4) | 10.7 (10.7) | 39.9 (39.3) | 13.7 (17.7) | 40.9 (43.0) |
| Rmerge (%)a | 12.3 (54.9) | 10.0 (85.5) | 9.1 (67.5) | 13.7 (64.9) | 23.6 (73.6) |
| I/s(I)a | 11.3 (8.6) | 15.6 (10.3) | 16.6 (10.7) | 13.9 (10.1) | 15.0 (11.2) |
| Completeness (%)a | 97.1 (87.3) | 99.6 (98.8) | 99.8 (99.8) | 99.8 (99.8) | 99.8 (100) |
| Refinement statistics | |||||
| Resolution range (Å) | 130.08–2.25 | 81.13–3.31 | 118.56–3.10 | — | 39.7–3.50 |
| Number of working sets/test sets | 45,717/2,438 | 6,065/295 | 7,709/371 | — | 5,467/260 |
| reflections | |||||
| Completeness (%) | 97.1 | 99.6 | 99.8 | — | 99.8 |
| Rwork (%)/Rfree (%) | 25.1/28.6 | 29.6/33.5 | 25.3/30.7 | — | 26.0/32.3 |
| Root-mean-square deviations | — | ||||
| Bond length (Å)/bond angles (°) | 0.008/1.5 | 0.007/1.32 | 0.011/1.6 | — | 0.007/1.3 |
| Ramachandran analysis (%) | — | ||||
| Favored | 95.7 | 91.3 | 90.3 | — | 89.9 |
| Allowed | 100 | 100 | 100 | — | 100 |
| Disallowed | 0 | 0 | 0 | — | 0 |
| Protein Data Bank accession no. | 5H5A | 5H5C | 5H54 | — | 5H55 |
| . | Mdm12dmLys . | Nonmodified Mdm12 . | |||
|---|---|---|---|---|---|
| . | +FOS-MEA10 . | Native . | SeMet . | Full-length (Mdm12FL) . | |
| Space group | P1 | P321 | P6122 | P6122 | P6122 |
| Unit cell parameters a, b, c (Å) | 42.87, 48.52, 130.13 | 93.39, 93.39, 81.13 | 136.90, 136.90, 76.56 | 137.17, 137.17, 76.62 | 137.17, 137.17, 76.62 |
| a, b, g (°) | 90.01, 91.50, 90.01 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 | 90.00, 90.00, 120.00 |
| Wavelength (Å) | 1 | 1 | 1 | 0.979 | 1 |
| Resolution range (Å) | 50-2.25 | 50-3.31 | 50-3.10 | 50-3.40 | 50-3.50 |
| Number of observed reflections | 527,803 | 535,031 | 1,608,644 | 977,533 | 235,394 |
| Redundancya | 3.8 (3.4) | 10.7 (10.7) | 39.9 (39.3) | 13.7 (17.7) | 40.9 (43.0) |
| Rmerge (%)a | 12.3 (54.9) | 10.0 (85.5) | 9.1 (67.5) | 13.7 (64.9) | 23.6 (73.6) |
| I/s(I)a | 11.3 (8.6) | 15.6 (10.3) | 16.6 (10.7) | 13.9 (10.1) | 15.0 (11.2) |
| Completeness (%)a | 97.1 (87.3) | 99.6 (98.8) | 99.8 (99.8) | 99.8 (99.8) | 99.8 (100) |
| Refinement statistics | |||||
| Resolution range (Å) | 130.08–2.25 | 81.13–3.31 | 118.56–3.10 | — | 39.7–3.50 |
| Number of working sets/test sets | 45,717/2,438 | 6,065/295 | 7,709/371 | — | 5,467/260 |
| reflections | |||||
| Completeness (%) | 97.1 | 99.6 | 99.8 | — | 99.8 |
| Rwork (%)/Rfree (%) | 25.1/28.6 | 29.6/33.5 | 25.3/30.7 | — | 26.0/32.3 |
| Root-mean-square deviations | — | ||||
| Bond length (Å)/bond angles (°) | 0.008/1.5 | 0.007/1.32 | 0.011/1.6 | — | 0.007/1.3 |
| Ramachandran analysis (%) | — | ||||
| Favored | 95.7 | 91.3 | 90.3 | — | 89.9 |
| Allowed | 100 | 100 | 100 | — | 100 |
| Disallowed | 0 | 0 | 0 | — | 0 |
| Protein Data Bank accession no. | 5H5A | 5H5C | 5H54 | — | 5H55 |
Values in parentheses are for the highest-resolution shell only.