Table 1.
Primer sequences for site-directed mutagenesis of HA3-K6a
SiteStrandMutagenic primer sequence (5′–3′)
S19A sense GCCGCCGGGGTTTCGCTGCCAACTCAGCC 
S19A antisense GGCTGAGTTGGCAGCGAAACCCCGGCGGC 
S22A sense GTTTCAGTGCCAACGCAGCCAGGCTCCCT 
S22A antisense AGGGAGCCTGGCTGCGTTGGCACTGAAAC 
S19A and S22A sense CGCCGGGGTTTCGCTGCCAACGCAGCCAGGCTCC 
S19A and S22A antisense GGAGCCTGGCTGCGTTGGCAGCGAAACCCCGGCG 
S37A sense CTTCAGCAGCGTCGCCGTGTCCCGCTC 
S37A antisense GAGCGGGACACGGCGACGCTGCTGAAG 
S60A sense CCAGGCCATACAGAGCGCGGCTGCCAAAGC 
S60A antisense GCTTTGGCAGCCGCGCTCTGTATGGCCTGG 
SiteStrandMutagenic primer sequence (5′–3′)
S19A sense GCCGCCGGGGTTTCGCTGCCAACTCAGCC 
S19A antisense GGCTGAGTTGGCAGCGAAACCCCGGCGGC 
S22A sense GTTTCAGTGCCAACGCAGCCAGGCTCCCT 
S22A antisense AGGGAGCCTGGCTGCGTTGGCACTGAAAC 
S19A and S22A sense CGCCGGGGTTTCGCTGCCAACGCAGCCAGGCTCC 
S19A and S22A antisense GGAGCCTGGCTGCGTTGGCAGCGAAACCCCGGCG 
S37A sense CTTCAGCAGCGTCGCCGTGTCCCGCTC 
S37A antisense GAGCGGGACACGGCGACGCTGCTGAAG 
S60A sense CCAGGCCATACAGAGCGCGGCTGCCAAAGC 
S60A antisense GCTTTGGCAGCCGCGCTCTGTATGGCCTGG 
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