Table 5.
Distribution of immunogold labeling of EJC-associated proteins at the nuclear membrane: nuclear side, middle, and cytoplasmic side
ProteinRatio nuclear/middleRatio cytoplasmic/middle
NXF1 3.89 (1.87–8.09) 3.89 (1.87–8.09) 
UPF2 3.71 (1.61–8.56) 3.43 (1.48–7.69) 
UPF3 3.08 (1.65–5.75) 2.08 (1.07–4.03) 
ProteinRatio nuclear/middleRatio cytoplasmic/middle
NXF1 3.89 (1.87–8.09) 3.89 (1.87–8.09) 
UPF2 3.71 (1.61–8.56) 3.43 (1.48–7.69) 
UPF3 3.08 (1.65–5.75) 2.08 (1.07–4.03) 

Gold particle counts, pooled over three experiments, were analyzed using a generalized linear model with Poisson distribution and a log link. The gold particle distribution ratios for the nuclear and cytoplasmic sides against the middle, together with 95% confidence intervals, are shown. The individual measurements are presented in Table S5. There is no significant difference in the gold particles/area unit ratios between proteins (likelihood ratio test, P < 0.68).

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