Table 1. Interlinked peptides of FtsY... | Rockefeller University Press

Table 1.

Interlinked peptides of FtsY with SecY(pBpa) identified by MS

Mass (MH⁺)/Da	Peptide of FtsY			Peptide of SecY			P value^{^a}	Intensity	Band^{^b}
Mass (MH⁺)/Da	Sequence^{^c}	Site	P value^{^d}	Sequence^{^c}	Site	P value^{^d}	P value^{^a}	Intensity	Band^{^b}
3011.5843	K.IITNLTEGASRK.Q	G255	4.4 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	6.2 × 10⁻⁷	2.7 × 10⁻¹²	1.3 × 10⁺⁶	×2
3801.921	R.DAEALYGLLKEEMGEILAK.V	K272	2.8 × 10⁻²	K.SGAFVPGIUPGEQTAK.Y	R357	4.0 × 10⁻⁸	1.2 × 10⁻⁹	1.1 × 10⁺⁷	×2
3817.9328	R.DAEALYGLLKEEM*GEILAK.V	K272	3.1 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	7.4 × 10⁻³	2.3 × 10⁻⁸	7.4 × 10⁺⁶	×2
3801.9419	R.DAEALYGLLKEEMGEILAK.V	L270	1.2 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	5.9 × 10⁻⁴	7.0 × 10⁻⁸	2.0 × 10⁺⁶	×2
3801.9302	R.DAEALYGLLKEEMGEILAK.V	L271	6.2 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	7.4 × 10⁻³	4.6 × 10⁻⁷	1.4 × 10⁺⁶	×2
3384.7577	K.GGVIFSVADQFGIPIR.Y	V456	4.8 × 10⁻⁷	K.SGAFVPGIUPGEQTAK.Y	R357	3.1 × 10⁻⁶	1.5 × 10⁻¹²	3.6 × 10⁺⁶	×1
3384.7606	K.GGVIFSVADQFGIPIR.Y	S459	4.0 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	6.5 × 10⁻¹⁰	2.6 × 10⁻¹⁵	3.6 × 10⁺⁶	×1
3384.7611	K.GGVIFSVADQFGIPIR.Y	F464	2.4 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	1.4 × 10⁻⁴	3.3 × 10⁻⁸	3.3 × 10⁺⁶	×1
2976.4774	K.ADDFIEALFAR.E	F488/I489	2.1 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	6.6 × 10⁻³	1.4 × 10⁻⁶	8.4 × 10⁺⁷	×1

Mass (MH⁺)/Da	Peptide of FtsY			Peptide of SecY			P value^{^a}	Intensity	Band^{^b}
Mass (MH⁺)/Da	Sequence^{^c}	Site	P value^{^d}	Sequence^{^c}	Site	P value^{^d}	P value^{^a}	Intensity	Band^{^b}
3011.5843	K.IITNLTEGASRK.Q	G255	4.4 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	6.2 × 10⁻⁷	2.7 × 10⁻¹²	1.3 × 10⁺⁶	×2
3801.921	R.DAEALYGLLKEEMGEILAK.V	K272	2.8 × 10⁻²	K.SGAFVPGIUPGEQTAK.Y	R357	4.0 × 10⁻⁸	1.2 × 10⁻⁹	1.1 × 10⁺⁷	×2
3817.9328	R.DAEALYGLLKEEM*GEILAK.V	K272	3.1 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	7.4 × 10⁻³	2.3 × 10⁻⁸	7.4 × 10⁺⁶	×2
3801.9419	R.DAEALYGLLKEEMGEILAK.V	L270	1.2 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	5.9 × 10⁻⁴	7.0 × 10⁻⁸	2.0 × 10⁺⁶	×2
3801.9302	R.DAEALYGLLKEEMGEILAK.V	L271	6.2 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	7.4 × 10⁻³	4.6 × 10⁻⁷	1.4 × 10⁺⁶	×2
3384.7577	K.GGVIFSVADQFGIPIR.Y	V456	4.8 × 10⁻⁷	K.SGAFVPGIUPGEQTAK.Y	R357	3.1 × 10⁻⁶	1.5 × 10⁻¹²	3.6 × 10⁺⁶	×1
3384.7606	K.GGVIFSVADQFGIPIR.Y	S459	4.0 × 10⁻⁶	K.SGAFVPGIUPGEQTAK.Y	R357	6.5 × 10⁻¹⁰	2.6 × 10⁻¹⁵	3.6 × 10⁺⁶	×1
3384.7611	K.GGVIFSVADQFGIPIR.Y	F464	2.4 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	1.4 × 10⁻⁴	3.3 × 10⁻⁸	3.3 × 10⁺⁶	×1
2976.4774	K.ADDFIEALFAR.E	F488/I489	2.1 × 10⁻⁴	K.SGAFVPGIUPGEQTAK.Y	R357	6.6 × 10⁻³	1.4 × 10⁻⁶	8.4 × 10⁺⁷	×1

Sites of cross-linking are underlined. SecYEG(pBpa) with pBpa incorporated at position 357 of SecY was expressed in vivo and purified after UV exposure of whole cells. Cross-linked FtsY peptides were analyzed as described in the Materials and methods. Residues 198–280 of FtsY correspond to the N domain, and residues 281–497 of FtsY correspond to the GTPase (G) domain.

P value for cross-linked peptide.

Bands from SDS gel at 130 kD (×1) and 160 kD (×2).

Underlined residues signify site of cross-link; M*, oxidized methionine.

Subscores per peptide.

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