| GFP-Rab GTPase . | Site of action (references) . | Percentage of PVs with internalized GFP-Rab . | |
|---|---|---|---|
| Toxoplasma . | N. caninum . | ||
| Rab1A | ER–Golgi (anterograde); intra-Golgi; IC-PM (Plutner et al., 1991; Tisdale et al., 1992; Moyer et al., 2001; Sannerud et al., 2006) | 95% | 33% |
| Rab2A | ER–Golgi (retrograde; Tisdale et al., 1992; Tisdale and Balch, 1996; Tisdale, 1999) | 67% | nd |
| Rab4A | EE-PM (fast; van der Sluijs et al., 1992; Daro et al., 1996; Vollenweider et al., 1997; McCaffrey et al., 2001) | 83% | nd |
| Rab5A | PM-EE (EE formation; Gorvel et al., 1991; Bucci et al., 1992; Stenmark et al., 1994) | 67% | nd |
| Rab6A | ER–Golgi; intra-Golgi; EE-TGN (Martinez et al., 1994; Girod et al., 1999; White et al., 1999; Mallard et al., 2002) | 46% | nd |
| Rab7A | EE-LE (EE maturation); LE-lysosome (Feng et al., 1995; Méresse et al., 1995; Vitelli et al., 1997; Press et al., 1998) | 62% | 22% |
| Rab9A | LE-TGN (Lombardi et al., 1993; Riederer et al., 1994) | 58% | nd |
| Rab10 | Vesicles to PM; ER structure/dynamics (Babbey et al., 2006; Sano et al., 2007, 2008; Schuck et al., 2007; Wang et al., 2010; Chen and Lippincott-Schwartz, 2013; English and Voeltz, 2013) | 90% | nd |
| Rab11A | RE-PM (slow); EE-TGN; TGN-PM; cytokinesis (Ullrich et al., 1996; Chen et al., 1998; Ren et al., 1998; Wilcke et al., 2000; Horgan et al., 2004; Wilson et al., 2005) | 89% | 31% |
| Rab11B | RE-PM (slow); EE-TGN; TGN-PM (Schlierf et al., 2000; Silvis et al., 2009; Butterworth et al., 2012) | 100% | 38% |
| Rab14 | TGN-endosomes; TGN-apical PM (Junutula et al., 2004; Kitt et al., 2008) | 94% | 29% |
| Rab24 | Autophagosome maturation/clearance; cell division (Munafó and Colombo, 2002; Militello et al., 2013; Ylä-Anttila et al., 2015) | 91% | 31% |
| Rab30 | Golgi morphology (Kelly et al., 2012) | 75% | 21% |
| Rab43 | ER–Golgi (Haas et al., 2007; Dejgaard et al., 2008) | 68% | 2% |
| GFP-Rab GTPase . | Site of action (references) . | Percentage of PVs with internalized GFP-Rab . | |
|---|---|---|---|
| Toxoplasma . | N. caninum . | ||
| Rab1A | ER–Golgi (anterograde); intra-Golgi; IC-PM (Plutner et al., 1991; Tisdale et al., 1992; Moyer et al., 2001; Sannerud et al., 2006) | 95% | 33% |
| Rab2A | ER–Golgi (retrograde; Tisdale et al., 1992; Tisdale and Balch, 1996; Tisdale, 1999) | 67% | nd |
| Rab4A | EE-PM (fast; van der Sluijs et al., 1992; Daro et al., 1996; Vollenweider et al., 1997; McCaffrey et al., 2001) | 83% | nd |
| Rab5A | PM-EE (EE formation; Gorvel et al., 1991; Bucci et al., 1992; Stenmark et al., 1994) | 67% | nd |
| Rab6A | ER–Golgi; intra-Golgi; EE-TGN (Martinez et al., 1994; Girod et al., 1999; White et al., 1999; Mallard et al., 2002) | 46% | nd |
| Rab7A | EE-LE (EE maturation); LE-lysosome (Feng et al., 1995; Méresse et al., 1995; Vitelli et al., 1997; Press et al., 1998) | 62% | 22% |
| Rab9A | LE-TGN (Lombardi et al., 1993; Riederer et al., 1994) | 58% | nd |
| Rab10 | Vesicles to PM; ER structure/dynamics (Babbey et al., 2006; Sano et al., 2007, 2008; Schuck et al., 2007; Wang et al., 2010; Chen and Lippincott-Schwartz, 2013; English and Voeltz, 2013) | 90% | nd |
| Rab11A | RE-PM (slow); EE-TGN; TGN-PM; cytokinesis (Ullrich et al., 1996; Chen et al., 1998; Ren et al., 1998; Wilcke et al., 2000; Horgan et al., 2004; Wilson et al., 2005) | 89% | 31% |
| Rab11B | RE-PM (slow); EE-TGN; TGN-PM (Schlierf et al., 2000; Silvis et al., 2009; Butterworth et al., 2012) | 100% | 38% |
| Rab14 | TGN-endosomes; TGN-apical PM (Junutula et al., 2004; Kitt et al., 2008) | 94% | 29% |
| Rab24 | Autophagosome maturation/clearance; cell division (Munafó and Colombo, 2002; Militello et al., 2013; Ylä-Anttila et al., 2015) | 91% | 31% |
| Rab30 | Golgi morphology (Kelly et al., 2012) | 75% | 21% |
| Rab43 | ER–Golgi (Haas et al., 2007; Dejgaard et al., 2008) | 68% | 2% |
HeLa cells were transiently transfected with GFP-Rab constructs and then infected with Toxoplasma or N. caninum for 24–32 h. The percentage of PVs with intravacuolar foci was determined as described in Materials and methods. Number of PVs: 19–28. EE, early endosome; IC, intermediate compartment; LE, late endosome; nd, not determined; PM, plasma membrane; RE, recycling endosome.