Table 3. Strains used in this study... | Rockefeller University Press

Table 3.

Strains used in this study

Strain	Genotype
JBY209	MATα kex2Δ2::hisG dap2::kan^r pep4::HIS3 ste13::LEU2
CB018	MATa KEX2⁺ pep4::HIS3 prb1::hisG prc1::hisG
BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
MAY22	BY4741 ADH1pr::VPS13-TAP pep4Δ::LEU2
MAY23	CBO18 ADH1pr::VPS13-HA
DC14	MATa his1
ELW55-2B	MATα cdc31-2ts ura3-52 trp1Δ
MDY1	JBY209 vps13Δ::URA3
MDY9	JBY209 cdc31-2ts
EBY43	MATa ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 GAL1pr-VPS13
JBY154-1D	MATα ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 vps13Δ::kan^r
MS 7902	CDC31⁺ leu2-3,112 ura3-52 ade2-101 his3-Δ300

Strain	Genotype
JBY209	MATα kex2Δ2::hisG dap2::kan^r pep4::HIS3 ste13::LEU2
CB018	MATa KEX2⁺ pep4::HIS3 prb1::hisG prc1::hisG
BY4741	MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0
MAY22	BY4741 ADH1pr::VPS13-TAP pep4Δ::LEU2
MAY23	CBO18 ADH1pr::VPS13-HA
DC14	MATa his1
ELW55-2B	MATα cdc31-2ts ura3-52 trp1Δ
MDY1	JBY209 vps13Δ::URA3
MDY9	JBY209 cdc31-2ts
EBY43	MATa ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 GAL1pr-VPS13
JBY154-1D	MATα ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 vps13Δ::kan^r
MS 7902	CDC31⁺ leu2-3,112 ura3-52 ade2-101 his3-Δ300

JBY209 (Brickner et al., 2001), CB018 (Brenner and Fuller, 1992), BY4741 (Brachmann et al., 1998), ELW55-2B (Weiss and Winey, 1996), JBY154-1D (Brickner and Fuller, 1997), and MS 7902 (Ivanovska and Rose, 2001) were constructed as described previously. MAY22, MAY23, MDY1, MDY9, and EBY43 were constructed as described in Materials and methods.

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