| Strain . | Genotype . |
|---|---|
| JBY209 | MATα kex2Δ2::hisG dap2::kanr pep4::HIS3 ste13::LEU2 |
| CB018 | MATa KEX2+ pep4::HIS3 prb1::hisG prc1::hisG |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| MAY22 | BY4741 ADH1pr::VPS13-TAP pep4Δ::LEU2 |
| MAY23 | CBO18 ADH1pr::VPS13-HA |
| DC14 | MATa his1 |
| ELW55-2B | MATα cdc31-2ts ura3-52 trp1Δ |
| MDY1 | JBY209 vps13Δ::URA3 |
| MDY9 | JBY209 cdc31-2ts |
| EBY43 | MATa ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 GAL1pr-VPS13 |
| JBY154-1D | MATα ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 vps13Δ::kanr |
| MS 7902 | CDC31+ leu2-3,112 ura3-52 ade2-101 his3-Δ300 |
| Strain . | Genotype . |
|---|---|
| JBY209 | MATα kex2Δ2::hisG dap2::kanr pep4::HIS3 ste13::LEU2 |
| CB018 | MATa KEX2+ pep4::HIS3 prb1::hisG prc1::hisG |
| BY4741 | MATa his3Δ1 leu2Δ0 met15Δ0 ura3Δ0 |
| MAY22 | BY4741 ADH1pr::VPS13-TAP pep4Δ::LEU2 |
| MAY23 | CBO18 ADH1pr::VPS13-HA |
| DC14 | MATa his1 |
| ELW55-2B | MATα cdc31-2ts ura3-52 trp1Δ |
| MDY1 | JBY209 vps13Δ::URA3 |
| MDY9 | JBY209 cdc31-2ts |
| EBY43 | MATa ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 GAL1pr-VPS13 |
| JBY154-1D | MATα ade2-1 can1-100 his3-11,15 leu2-3,-112 trp1-1 ura3-1 vps13Δ::kanr |
| MS 7902 | CDC31+ leu2-3,112 ura3-52 ade2-101 his3-Δ300 |
JBY209 (Brickner et al., 2001), CB018 (Brenner and Fuller, 1992), BY4741 (Brachmann et al., 1998), ELW55-2B (Weiss and Winey, 1996), JBY154-1D (Brickner and Fuller, 1997), and MS 7902 (Ivanovska and Rose, 2001) were constructed as described previously. MAY22, MAY23, MDY1, MDY9, and EBY43 were constructed as described in Materials and methods.