| Test lipid | Bound |
|---|---|
| % | |
| N domain | |
| None | 2.0 ± 0.8 |
| PA | 48.0 ± 2.0 |
| PI(4)P | 19.0 ± 3.4 |
| PI(4,5)P2 | 31.0 ± 2.5 |
| PI(3)P | 3.0 ± 1.3 |
| PI(5)P | 2.0 ± 0.7 |
| C domain | |
| None | 2.0 ± 1.1 |
| PA | 6.0 ± 1.4 |
| PI(4)P | 11.0 ± 4.4 |
| PI(4,5)P2 | 41.0 ± 2.5 |
| PI(3)P | 8.0 ± 0.9 |
| PI(5)P | 7.0 ± 1.6 |
| Test lipid | Bound |
|---|---|
| N domain | |
| None | 2.0 ± 0.8 |
| PA | 48.0 ± 2.0 |
| PI(4)P | 19.0 ± 3.4 |
| PI(4,5)P2 | 31.0 ± 2.5 |
| PI(3)P | 3.0 ± 1.3 |
| PI(5)P | 2.0 ± 0.7 |
| C domain | |
| None | 2.0 ± 1.1 |
| PA | 6.0 ± 1.4 |
| PI(4)P | 11.0 ± 4.4 |
| PI(4,5)P2 | 41.0 ± 2.5 |
| PI(3)P | 8.0 ± 0.9 |
| PI(5)P | 7.0 ± 1.6 |
The percentage of the N domain or C domain bound was calculated as the top fraction divided by the sum of the pellet and bottom fraction multiplied by 100. Shown are mean ± SD values from three independent experiments.