Quantification of liposome-binding assays with full-length Vps13p
Test lipid
.
Bound
.
%
None
3.6 ± 1.3
PA
26.6 ± 2.5
LPA
13.7 ± 2.5
PI(3)P
17.7 ± 3.0
PI(4)P
20.0 ± 1.7
PI(5)P
15.2 ± 0.3
PI(3,5)P2
6.5 ± 2.5
PI(3,4)P2
7.8 ± 2.1
PI(4,5)P2
20.5 ± 0.5
PS
5.8 ± 1.1
PI
2 ± 1.5
Test lipid
.
Bound
.
%
None
3.6 ± 1.3
PA
26.6 ± 2.5
LPA
13.7 ± 2.5
PI(3)P
17.7 ± 3.0
PI(4)P
20.0 ± 1.7
PI(5)P
15.2 ± 0.3
PI(3,5)P2
6.5 ± 2.5
PI(3,4)P2
7.8 ± 2.1
PI(4,5)P2
20.5 ± 0.5
PS
5.8 ± 1.1
PI
2 ± 1.5
The percentage of Vps13p bound was calculated as the top fraction divided by the sum of the pellet and bottom fraction multiplied by 100. Shown are mean ± SD values from three independent experiments.