Table 1.
Strains used in this study
StrainGenotype
YMO400 W303-1A, ADE2, HIS3, VPH1:: VPH1-EGFP (CgLEU2) 
YMO411 W303-1A, ADE2, HIS3, leu2::VPH1-EGFP (LEU2) 
YMO412 W303-1A, ADE2, pep4Δ::SpHIS5 (equivalent to ScHIS3), leu2::VPH1-EGFP (LEU2) 
YMO413 W303-1A, ADE2, atg1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO414 W303-1A, ADE2, atg2Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO415 W303-1A, ADE2, atg3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO416 W303-1A, ADE2, atg4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO417 W303-1A, ADE2, atg5Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO418 W303-1A, ADE2, atg6Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO419 W303-1A, ADE2, atg7Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO420 W303-1A, ADE2, atg8Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO421 W303-1A, ADE2, atg9Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO422 W303-1A, ADE2, atg10Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO423 W303-1A, ADE2, atg11Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO424 W303-1A, ADE2, atg12Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO425 W303-1A, ADE2,atg13Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO426 W303-1A, ADE2, atg14Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO427 W303-1A, ADE2, atg15Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO428 W303-1A, ADE2, atg16Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO429 W303-1A, ADE2, atg17Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO430 W303-1A, ADE2, atg18Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO431 W303-1A, ADE2, ego1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO432 W303-1A, ADE2, ego3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO433 W303-1A, ADE2, nvj1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO434 W303-1A, ADE2, vac8Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO435 W303-1A, ADE2, vtc3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO436 W303-1A, ADE2, vtc4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO437 W303-1A, ADE2, vps4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO438 W303-1A, ADE2, vps27Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO439 W303-1A, ADE2, hse1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO440 W303-1A, ADE2, vps23Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO441 W303-1A, ADE2, vps28Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO442 W303-1A, ADE2, vps25Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO443 W303-1A, ADE2,vps36Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO444 W303-1A, ADE2, snf7Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO445 W303-1A, ADE2, doa4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO446 YMO438, ura3::pRS306, URA3 
YMO447 YMO438, ura3::VPS27WT-pRS306(URA3) 
YMO448 YMO438, ura3::vps27VHSmut-pRS306(URA3) 
YMO459 YMO438, ura3::vps27FYVEmut-pRS306(URA3) 
YMO450 YMO438, ura3::vps27UIMmut-pRS306(URA3) 
YMO451 YMO438, ura3::vps27PTAPLmut-pRS306(URA3) 
YMO452 YMO438, ura3::vps27ΔCB-pRS306(URA3) 
YMO453 W303-1A, ADE2, chc1-5::HIS3, leu2::VPH1-EGFP (LEU2) 
YMO454 W303-1A, ADE2, vps18Δ::SpHIS5, leu2::VPH1-EGFP (LEU2), ura3::vps18ts–pRS306(URA3) 
YMO455 YMO438, ura3::HA-VPS27WT-pRS306(URA3) 
YMO456 YMO438, ura3::HA-vps27VHSmut-pRS306(URA3) 
YMO457 YMO438, ura3::HA-vps27FYVEmut-pRS306(URA3) 
YMO458 YMO438, ura3::HA-vps27UIMmut-pRS306(URA3) 
YMO459 YMO438, ura3::HA-vps27PTAPLmut-pRS306(URA3) 
YMO460 YMO438, ura3::HA-vps27ΔCB-pRS306(URA3) 
YMO461 W303-1A, ADE2, HIS3, PHO8:: PCUP1 yEGFP-PHO8 (CgLEU2) 
YMO462 W303-1A, ADE2, pep4Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2) 
YMO463 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2), ura3::pRS306(URA3) 
YMO464 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2), ura3::VPS27WT-pRS306(URA3) 
YMO465 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 -yEGFP-PHO8 (CgLEU2), ura3::vps27ΔCB-pRS306(URA3) 
YMO471 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2) 
YMO472 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2), ura3::VPS27WT-pRS306(URA3) 
YMO473 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2), ura3::vps27ΔCB-pRS306(URA3) 
YMO481 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-VPS27WT-pRS306(URA3) 
YMO482 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-vps27FYVEmut-pRS306(URA3) 
YMO483 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-vps27FYVEΔCB–pRS306(URA3) 
YMO501 W303-1A, ADE2+, HIS3+, OSW5::OSW5-EGFP (CgLEU2) 
YMO502 W303-1A, ADE2+, pep4Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) 
YMO503 W303-1A, ADE2+, atg1Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) 
YMO504 W303-1A, ADE2+, vps27Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) ura3::VPS27WT-pRS306(URA3) 
YMO505 W303-1A, ADE2+, vps27Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2), ura3::vps27DCB-pRS306(URA3) 
YYK101 W303-1A, ADE2+, OSW5::OSW5-EGFP (CgLEU2), ERG6-mCherry-KanMX 
YYK102 W303-1A, ADE2+, OSW5::FAA4-EGFP (CgLEU2), ERG6-mCherry-KanMX 
StrainGenotype
YMO400 W303-1A, ADE2, HIS3, VPH1:: VPH1-EGFP (CgLEU2) 
YMO411 W303-1A, ADE2, HIS3, leu2::VPH1-EGFP (LEU2) 
YMO412 W303-1A, ADE2, pep4Δ::SpHIS5 (equivalent to ScHIS3), leu2::VPH1-EGFP (LEU2) 
YMO413 W303-1A, ADE2, atg1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO414 W303-1A, ADE2, atg2Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO415 W303-1A, ADE2, atg3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO416 W303-1A, ADE2, atg4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO417 W303-1A, ADE2, atg5Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO418 W303-1A, ADE2, atg6Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO419 W303-1A, ADE2, atg7Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO420 W303-1A, ADE2, atg8Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO421 W303-1A, ADE2, atg9Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO422 W303-1A, ADE2, atg10Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO423 W303-1A, ADE2, atg11Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO424 W303-1A, ADE2, atg12Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO425 W303-1A, ADE2,atg13Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO426 W303-1A, ADE2, atg14Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO427 W303-1A, ADE2, atg15Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO428 W303-1A, ADE2, atg16Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO429 W303-1A, ADE2, atg17Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO430 W303-1A, ADE2, atg18Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO431 W303-1A, ADE2, ego1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO432 W303-1A, ADE2, ego3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO433 W303-1A, ADE2, nvj1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO434 W303-1A, ADE2, vac8Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO435 W303-1A, ADE2, vtc3Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO436 W303-1A, ADE2, vtc4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO437 W303-1A, ADE2, vps4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO438 W303-1A, ADE2, vps27Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO439 W303-1A, ADE2, hse1Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO440 W303-1A, ADE2, vps23Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO441 W303-1A, ADE2, vps28Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO442 W303-1A, ADE2, vps25Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO443 W303-1A, ADE2,vps36Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO444 W303-1A, ADE2, snf7Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO445 W303-1A, ADE2, doa4Δ::SpHIS5, leu2::VPH1-EGFP (LEU2) 
YMO446 YMO438, ura3::pRS306, URA3 
YMO447 YMO438, ura3::VPS27WT-pRS306(URA3) 
YMO448 YMO438, ura3::vps27VHSmut-pRS306(URA3) 
YMO459 YMO438, ura3::vps27FYVEmut-pRS306(URA3) 
YMO450 YMO438, ura3::vps27UIMmut-pRS306(URA3) 
YMO451 YMO438, ura3::vps27PTAPLmut-pRS306(URA3) 
YMO452 YMO438, ura3::vps27ΔCB-pRS306(URA3) 
YMO453 W303-1A, ADE2, chc1-5::HIS3, leu2::VPH1-EGFP (LEU2) 
YMO454 W303-1A, ADE2, vps18Δ::SpHIS5, leu2::VPH1-EGFP (LEU2), ura3::vps18ts–pRS306(URA3) 
YMO455 YMO438, ura3::HA-VPS27WT-pRS306(URA3) 
YMO456 YMO438, ura3::HA-vps27VHSmut-pRS306(URA3) 
YMO457 YMO438, ura3::HA-vps27FYVEmut-pRS306(URA3) 
YMO458 YMO438, ura3::HA-vps27UIMmut-pRS306(URA3) 
YMO459 YMO438, ura3::HA-vps27PTAPLmut-pRS306(URA3) 
YMO460 YMO438, ura3::HA-vps27ΔCB-pRS306(URA3) 
YMO461 W303-1A, ADE2, HIS3, PHO8:: PCUP1 yEGFP-PHO8 (CgLEU2) 
YMO462 W303-1A, ADE2, pep4Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2) 
YMO463 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2), ura3::pRS306(URA3) 
YMO464 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1-yEGFP-PHO8 (CgLEU2), ura3::VPS27WT-pRS306(URA3) 
YMO465 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 -yEGFP-PHO8 (CgLEU2), ura3::vps27ΔCB-pRS306(URA3) 
YMO471 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2) 
YMO472 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2), ura3::VPS27WT-pRS306(URA3) 
YMO473 W303-1A, ADE2, vps27Δ::SpHIS5, PHO8:: PCUP1 yEGFP-CPS1 (CgLEU2), ura3::vps27ΔCB-pRS306(URA3) 
YMO481 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-VPS27WT-pRS306(URA3) 
YMO482 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-vps27FYVEmut-pRS306(URA3) 
YMO483 W303-1A, ADE2, vps27Δ::SpHIS5, ura3::PVPS27 Envy-vps27FYVEΔCB–pRS306(URA3) 
YMO501 W303-1A, ADE2+, HIS3+, OSW5::OSW5-EGFP (CgLEU2) 
YMO502 W303-1A, ADE2+, pep4Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) 
YMO503 W303-1A, ADE2+, atg1Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) 
YMO504 W303-1A, ADE2+, vps27Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2) ura3::VPS27WT-pRS306(URA3) 
YMO505 W303-1A, ADE2+, vps27Δ::SpHIS5, OSW5::OSW5-EGFP (CgLEU2), ura3::vps27DCB-pRS306(URA3) 
YYK101 W303-1A, ADE2+, OSW5::OSW5-EGFP (CgLEU2), ERG6-mCherry-KanMX 
YYK102 W303-1A, ADE2+, OSW5::FAA4-EGFP (CgLEU2), ERG6-mCherry-KanMX 
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