Table 1. Antibodies used to detect the... | Rockefeller University Press

Table 1.

Antibodies used to detect the somatic H1 subtypes and their application

Antibody	ESCs, immunofluorescence	PGCs		ESCs and PGCs, Western blotting	Embryos, immunofluorescence
Antibody	ESCs, immunofluorescence	Cryosections	Paraffin sections	ESCs and PGCs, Western blotting	Embryos, immunofluorescence
Rb Flag F7425	1:300	1:100	Not tested	1:1000	Not tested
Ms HA 12CA5	1:100	NA	Not tested	1:1000	1:200
Ms H1.0 sc-56695	Not tested	NA	1:100	1:1000	Not tested
Rb H1x ab31972	1:100	1:100	1:100	1:1000	1:100
H1foo (K14) sc-99918	Not tested	NA	NA	Not tested	Not tested

Antibody	ESCs, immunofluorescence	PGCs		ESCs and PGCs, Western blotting	Embryos, immunofluorescence
Antibody	ESCs, immunofluorescence	Cryosections	Paraffin sections	ESCs and PGCs, Western blotting	Embryos, immunofluorescence
Rb Flag F7425	1:300	1:100	Not tested	1:1000	Not tested
Ms HA 12CA5	1:100	NA	Not tested	1:1000	1:200
Ms H1.0 sc-56695	Not tested	NA	1:100	1:1000	Not tested
Rb H1x ab31972	1:100	1:100	1:100	1:1000	1:100
H1foo (K14) sc-99918	Not tested	NA	NA	Not tested	Not tested

NA, no specific signal under the conditions tested.