| Construct . | Calculated molecular massa . | Observed molecular massb . | s20,w . | |
|---|---|---|---|---|
| . | Monomer . | Dimer . | . | . |
| kD | kD | kD | ||
| Myo51 | 276 | 552 | 390 ± 12 (n = 6) | 9.6 (n = 2)c |
| Myo51–Rng8/9 | 346 | 692 | 301 ±10 (n = 6) | 7.5 (n = 2) |
| Construct . | Calculated molecular massa . | Observed molecular massb . | s20,w . | |
|---|---|---|---|---|
| . | Monomer . | Dimer . | . | . |
| kD | kD | kD | ||
| Myo51 | 276 | 552 | 390 ± 12 (n = 6) | 9.6 (n = 2)c |
| Myo51–Rng8/9 | 346 | 692 | 301 ±10 (n = 6) | 7.5 (n = 2) |
The calculated molecular masses include the tags, light chains, and one copy each of Rng8 and Rng9 when present.
The molecular mass values (mean ± SD) were obtained from two independent protein preparations.
Mean s20,w from two independent preparations. The values obtained from the two experiments were the same.