Table 1.
Functional screening of the effect of RhoA, RhoB, and RhoC siRNAs on the barrier properties of human primary endothelial cells
Barrier analysis/siRNARhoARhoBRhoCRhoA/B/C
Unstimulated HUVECs     
Constitutive barrier function −/+ + ++ +++ 
F-actin levels − − − +++ 
TNF-stimulated HUVECs     
TNF-induced barrier dysfunction − − − ND 
TNF-induced F-actin increase − − − +++ 
Leukocyte transendothelial migration (static) − − − − 
Acute cell contraction −/+ + −/+ +++ 
Barrier restoration after contraction − +++ −/+ +++ 
Barrier analysis/siRNARhoARhoBRhoCRhoA/B/C
Unstimulated HUVECs     
Constitutive barrier function −/+ + ++ +++ 
F-actin levels − − − +++ 
TNF-stimulated HUVECs     
TNF-induced barrier dysfunction − − − ND 
TNF-induced F-actin increase − − − +++ 
Leukocyte transendothelial migration (static) − − − − 
Acute cell contraction −/+ + −/+ +++ 
Barrier restoration after contraction − +++ −/+ +++ 

ND, not determined.

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