| Antibody . | Raised in or recognizes . | Source . | Concentration . | Incubation . |
|---|---|---|---|---|
| Primary | ||||
| FLASH | Rabbit | Yang et al., 2009 | 1:2,000 | 4°C, overnight |
| V5 | Mouse | Invitrogen | 1:1,000 | 4°C, overnight |
| Mxc | Guinea pig | White et al., 2011 | 1:2,000 | 4°C, overnight |
| Mute | Guinea pig | Bulchand et al., 2010 | 1:5,000 | 4°C, overnight |
| Lsm10 | Rabbit | Liu et al., 2006 | 1:2,000 | 4°C, overnight |
| Lsm11 | Rabbit | Liu et al., 2006 | 1:2,000 | 4°C, overnight |
| HP1 | Mouse | Developmental Studies Hybridoma Bank | 1:1,000 | 4°C, overnight |
| H2aV | Rabbit | Leach et al., 2000 | 1:1,000 | 4°C, overnight |
| GFP | Chicken | EMD Millipore | 1:1,000 | 4°C, overnight |
| β-Tubulin | Rabbit | Abcam | 1:5,000 | Room temperature, 2 h |
| Secondary | ||||
| Alexa Fluor 488 | Rabbit | Invitrogen | 1:2,000 | Room temperature, 2 h |
| Alexa Fluor 488 | Mouse IgG2a | Invitrogen | 1:1,000 | Room temperature, 2 h |
| Cy3 | guinea pig | Jackson ImmunoResearch Laboratories, Inc. | 1:1,000 | Room temperature, 2 h |
| Alexa Fluor 555 | Mouse IgG1 | Invitrogen | 1:1,000 | Room temperature, 2 h |
| Cy5 | rabbit | Abcam | 1:2,000 | Room temperature, 2 h |
| Cy5 | rabbit | Jackson ImmunoResearch Laboratories, Inc. | 1:1,000 | Room temperature, 2 h |
| ECL donkey HRP | rabbit | GE Healthcare | 1:10,000 | Room temperature, 1 h |
| ECL prime donkey HRP | mouse | GE Healthcare | 1:10,000 | Room temperature, 1 h |
| Antibody . | Raised in or recognizes . | Source . | Concentration . | Incubation . |
|---|---|---|---|---|
| Primary | ||||
| FLASH | Rabbit | Yang et al., 2009 | 1:2,000 | 4°C, overnight |
| V5 | Mouse | Invitrogen | 1:1,000 | 4°C, overnight |
| Mxc | Guinea pig | White et al., 2011 | 1:2,000 | 4°C, overnight |
| Mute | Guinea pig | Bulchand et al., 2010 | 1:5,000 | 4°C, overnight |
| Lsm10 | Rabbit | Liu et al., 2006 | 1:2,000 | 4°C, overnight |
| Lsm11 | Rabbit | Liu et al., 2006 | 1:2,000 | 4°C, overnight |
| HP1 | Mouse | Developmental Studies Hybridoma Bank | 1:1,000 | 4°C, overnight |
| H2aV | Rabbit | Leach et al., 2000 | 1:1,000 | 4°C, overnight |
| GFP | Chicken | EMD Millipore | 1:1,000 | 4°C, overnight |
| β-Tubulin | Rabbit | Abcam | 1:5,000 | Room temperature, 2 h |
| Secondary | ||||
| Alexa Fluor 488 | Rabbit | Invitrogen | 1:2,000 | Room temperature, 2 h |
| Alexa Fluor 488 | Mouse IgG2a | Invitrogen | 1:1,000 | Room temperature, 2 h |
| Cy3 | guinea pig | Jackson ImmunoResearch Laboratories, Inc. | 1:1,000 | Room temperature, 2 h |
| Alexa Fluor 555 | Mouse IgG1 | Invitrogen | 1:1,000 | Room temperature, 2 h |
| Cy5 | rabbit | Abcam | 1:2,000 | Room temperature, 2 h |
| Cy5 | rabbit | Jackson ImmunoResearch Laboratories, Inc. | 1:1,000 | Room temperature, 2 h |
| ECL donkey HRP | rabbit | GE Healthcare | 1:10,000 | Room temperature, 1 h |
| ECL prime donkey HRP | mouse | GE Healthcare | 1:10,000 | Room temperature, 1 h |