The data for each of the genetic rescue experiments presented as circle charts in Figs. 1, 2, and 3 is shown. For the transgenic rescue crosses, a recombinant chromosome containing FLASH^Df and a FLASH transgene was provided maternally. For each experiment, a χ² analysis was performed under the null hypothesis that the phenotypic classes of adult flies were present in expected Mendelian ratios (two thirds CyO/mutant control siblings and one third homozygous mutant genotype). A Student’s t test was used to determine whether the number of flies in the experiment class was statistically different from when no transgene was present in the FLASH^PBac/Df background. Note that three of the transgenes, FLASH^NL125, FLASH ^78–844, and FLASH^LDIY45,71, behaved as dominant negatives, consistent with them being incorporated into the HLB and competing with the small amount of endogenous FLASH. The FLASH^PBac/PBac phenotype suggests the presence of a second site mutation on the PBac chromosome. Assessment of fertility for each genotype is also noted. The last column indicates transgenic FLASH proteins capable of localizing to the HLB. N, no; n/a, not applicable; n/d, not determined; Y, yes.