| . | REF . | mex67-5 . |
|---|---|---|
| Particles tracked | 291 | 203 |
| Nuclear docking events | 47 | 23 |
| Export events | 43 | 9 |
| Retrograde export events | 0 | 7 |
| Mean export time—dwell time analysis (ms) | 188a | ND |
| Mean export time—MLE (ms) | 215a | ND |
| Mean nuclear docking time during export—dwell time analysis (ms) | 32a | 362a |
| Mean nuclear docking time during export—MLE (ms) | 39a | 202a |
| Mean transition time during export—dwell time analysis (ms) | 87a | 406a |
| Mean transition time during export—MLE (ms) | 99a | 383a |
| Mean cytoplasmic docking time during export—dwell time analysis (ms) | 62a | 1,258a |
| Mean cytoplasmic docking time during export—MLE (ms) | 77a | 943a |
| NE-associated mRNPs per cell | 0.2 ± 0.4b | 0.5 ± 0.7b |
| Nuclear scanning events | 4 | 2 |
| Cytoplasmic scanning events | 102 | 16 |
| . | REF . | mex67-5 . |
|---|---|---|
| Particles tracked | 291 | 203 |
| Nuclear docking events | 47 | 23 |
| Export events | 43 | 9 |
| Retrograde export events | 0 | 7 |
| Mean export time—dwell time analysis (ms) | 188a | ND |
| Mean export time—MLE (ms) | 215a | ND |
| Mean nuclear docking time during export—dwell time analysis (ms) | 32a | 362a |
| Mean nuclear docking time during export—MLE (ms) | 39a | 202a |
| Mean transition time during export—dwell time analysis (ms) | 87a | 406a |
| Mean transition time during export—MLE (ms) | 99a | 383a |
| Mean cytoplasmic docking time during export—dwell time analysis (ms) | 62a | 1,258a |
| Mean cytoplasmic docking time during export—MLE (ms) | 77a | 943a |
| NE-associated mRNPs per cell | 0.2 ± 0.4b | 0.5 ± 0.7b |
| Nuclear scanning events | 4 | 2 |
| Cytoplasmic scanning events | 102 | 16 |
Export data underlying dwell time analysis may not have a normal distribution; consequently, an MLE analysis was performed (Kay, 1993). The differences between dwell times in REF and mex67-5 strains were tested using the distribution independent Wilcoxon rank-sum test and were found to be significant at P < 0.05 for the transition and cytoplasmic docking states. For more details and a discussion of error measurements, see the Materials and methods section Statistical analyses.
Standard deviation.