Table 2.
Percent of directed motion of pericentric chromatin
Minimum displacementUntreated (n = 22 foci)Benomyl (n = 16 foci)kMT dynamics from mitotic simulationa
ParallelPerpendicularParallelPerpendicular
nm       
300 19% 4%  0% 0% 20% 
350 16% 1%  0% 0% 14% 
400 11% 0%  0% 0% 9% 
Minimum displacementUntreated (n = 22 foci)Benomyl (n = 16 foci)kMT dynamics from mitotic simulationa
ParallelPerpendicularParallelPerpendicular
nm       
300 19% 4%  0% 0% 20% 
350 16% 1%  0% 0% 14% 
400 11% 0%  0% 0% 9% 
a

Simulated kMT dynamics were generated using the default settings of a MATLAB Mitotic Spindle Simulation that incorporates kMT dynamics with a chromatin spring to recapitulate lacO fluctuation and stretching in WT and mutant cells (Stephens et al., 2013).

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