| Parameters analyzed . | Control (NT) . | A2 WT . | A2 K286A . | WA . | A2 WT + WA . | A2 K286A +WA . |
|---|---|---|---|---|---|---|
| Number of cells | 56 | 25 | 32 | 39 | 25 | 25 |
| Number of spikes (total) | 1,322 | 551 | 358 | 1,136 | 702 | 304 |
| Number of spikes per cell | 23 ± 1 | 22 ± 2 | 11 ± 1 | 29 ± 3 | 28 ± 3 | 12 ± 1 |
| Number of spikes (analyzed) | 1,021 | 499 | 275 | 933 | 638 | 217 |
| Imax (pA) | 61.1 ± 2.9 | 61.2 ± 2.4 | 50.4 ± 1.9 | 79.6 ± 2.0 | 73.4 ± 3.1 | 52.2 ± 2.3 |
| Half width (ms) | 28.2 ± 4.2 | 30.1 ± 4.1 | 46.8 ± 2.9 | 21.6 ± 2.6 | 22.5 ± 3.3 | 47.8 ± 3.1 |
| Q (fC) | 1780 ± 170 | 1770 ± 80 | 1750 ± 120 | 1790 ± 90 | 1720 ± 120 | 1790 ± 50 |
| Slope (pA/ms) | 6.1 ± 0.9 | 5.8 ± 0.9 | 2.9 ± 0.8 | 10.9 ± 0.7 | 11.2 ± 1.5 | 3.1 ± 0.7 |
| Rise time (ms) | 10.2 ± 1.8 | 11.5 ± 1.5 | 18.1± 1.2 | 7.1± 1.1 | 7.1± 1.1 | 19.1 ± 2.1 |
| Decay time (ms) | 58.9 ± 5.9 | 60.9 ± 3.5 | 65.8 ± 2.2 | 35.7 ± 2.0 | 38.8 ± 3.2 | 64.1 ± 4.0 |
| Spike with foot (%) | 29 | 26 | 32 | 17 | 16 | 27 |
| Number of feet analyzed | 304 | 129 | 89 | 159 | 102 | 59 |
| Number of feet per cell | 5.4 ± 0.9 | 5.1 ± 0.9 | 2.7 ± 0.8 | 4.0 ± 0.4 | 4.0 ± 0.8 | 2.4 ± 0.7 |
| I foot (pA) | 4.4 ± 0.6 | 4.4 ± 0.8 | 4.5 ± 0.7 | 5.4 ± 0.7 | 4.9 ± 0.7 | 4.1 ± 0.5 |
| Q foot (fC) | 20.8 ± 2.1 | 20.8 ± 2.3 | 31.3 ± 3.4 | 15.3 ± 4.4 | 14.3 ± 3.4 | 30.6 ± 1.9 |
| t foot(ms) | 14.7 ± 2.1 | 15.7 ± 1.9 | 24.3 ± 2.1 | 9.3 ± 2.5 | 8.3 ± 2.1 | 23.5 ± 0.9 |
| Parameters analyzed . | Control (NT) . | A2 WT . | A2 K286A . | WA . | A2 WT + WA . | A2 K286A +WA . |
|---|---|---|---|---|---|---|
| Number of cells | 56 | 25 | 32 | 39 | 25 | 25 |
| Number of spikes (total) | 1,322 | 551 | 358 | 1,136 | 702 | 304 |
| Number of spikes per cell | 23 ± 1 | 22 ± 2 | 11 ± 1 | 29 ± 3 | 28 ± 3 | 12 ± 1 |
| Number of spikes (analyzed) | 1,021 | 499 | 275 | 933 | 638 | 217 |
| Imax (pA) | 61.1 ± 2.9 | 61.2 ± 2.4 | 50.4 ± 1.9 | 79.6 ± 2.0 | 73.4 ± 3.1 | 52.2 ± 2.3 |
| Half width (ms) | 28.2 ± 4.2 | 30.1 ± 4.1 | 46.8 ± 2.9 | 21.6 ± 2.6 | 22.5 ± 3.3 | 47.8 ± 3.1 |
| Q (fC) | 1780 ± 170 | 1770 ± 80 | 1750 ± 120 | 1790 ± 90 | 1720 ± 120 | 1790 ± 50 |
| Slope (pA/ms) | 6.1 ± 0.9 | 5.8 ± 0.9 | 2.9 ± 0.8 | 10.9 ± 0.7 | 11.2 ± 1.5 | 3.1 ± 0.7 |
| Rise time (ms) | 10.2 ± 1.8 | 11.5 ± 1.5 | 18.1± 1.2 | 7.1± 1.1 | 7.1± 1.1 | 19.1 ± 2.1 |
| Decay time (ms) | 58.9 ± 5.9 | 60.9 ± 3.5 | 65.8 ± 2.2 | 35.7 ± 2.0 | 38.8 ± 3.2 | 64.1 ± 4.0 |
| Spike with foot (%) | 29 | 26 | 32 | 17 | 16 | 27 |
| Number of feet analyzed | 304 | 129 | 89 | 159 | 102 | 59 |
| Number of feet per cell | 5.4 ± 0.9 | 5.1 ± 0.9 | 2.7 ± 0.8 | 4.0 ± 0.4 | 4.0 ± 0.8 | 2.4 ± 0.7 |
| I foot (pA) | 4.4 ± 0.6 | 4.4 ± 0.8 | 4.5 ± 0.7 | 5.4 ± 0.7 | 4.9 ± 0.7 | 4.1 ± 0.5 |
| Q foot (fC) | 20.8 ± 2.1 | 20.8 ± 2.3 | 31.3 ± 3.4 | 15.3 ± 4.4 | 14.3 ± 3.4 | 30.6 ± 1.9 |
| t foot(ms) | 14.7 ± 2.1 | 15.7 ± 1.9 | 24.3 ± 2.1 | 9.3 ± 2.5 | 8.3 ± 2.1 | 23.5 ± 0.9 |
The expression of AnxA2-K286A-GFP and WA treatment significantly affected various amperometric signal parameters when catecholamine release was evoked after the application of 100 mM KCl for 10 s.