| Cell type/segment . | BLM+/− Ch14q (320 kb) . | BLM−/− Ch14q (320 kb) . | BLM+/− Ch14q PhenDC3 (320 kb) . | BLM−/− Ch14q PhenDC3 (320 kb) . | BLM+/− Igh PhenDC3 (235 kb) . | BLM−/− Igh PhenDC3 (235 kb) . |
|---|---|---|---|---|---|---|
| NR | 48 | 50 | 71 | 49 | 55 | 57 |
| NG | 56 | 45 | 68 | 57 | 82 | 62 |
| NRG | 32 | 23 | 43 | 27 | 38 | 36 |
| Total forks | 38 | 37 | 50 | 53 | 38 | 36 |
| NRG100 | 9 | 8 | 11 | 13 | – | – |
| Total forks100 | 10 | 11 | 13 | 16 | – | – |
| Average fork speed (kb/min; full segment) | 2.95 ± 0.17 | 2.54 ± 0.12 | 3.00 ± 0.06 | 2.01 ± 0.14 | 2.73 ± 0.49 | 2.59 ± 0.1 |
| Average fork speed (kb/min; terminal 100 kb) | 3.50 ± 0.24 | 2.67 ± 0.13 | 3.61 ± 0.07 | 2.06 ± 0.15 | – | – |
| Cell type/segment . | BLM+/− Ch14q (320 kb) . | BLM−/− Ch14q (320 kb) . | BLM+/− Ch14q PhenDC3 (320 kb) . | BLM−/− Ch14q PhenDC3 (320 kb) . | BLM+/− Igh PhenDC3 (235 kb) . | BLM−/− Igh PhenDC3 (235 kb) . |
|---|---|---|---|---|---|---|
| NR | 48 | 50 | 71 | 49 | 55 | 57 |
| NG | 56 | 45 | 68 | 57 | 82 | 62 |
| NRG | 32 | 23 | 43 | 27 | 38 | 36 |
| Total forks | 38 | 37 | 50 | 53 | 38 | 36 |
| NRG100 | 9 | 8 | 11 | 13 | – | – |
| Total forks100 | 10 | 11 | 13 | 16 | – | – |
| Average fork speed (kb/min; full segment) | 2.95 ± 0.17 | 2.54 ± 0.12 | 3.00 ± 0.06 | 2.01 ± 0.14 | 2.73 ± 0.49 | 2.59 ± 0.1 |
| Average fork speed (kb/min; terminal 100 kb) | 3.50 ± 0.24 | 2.67 ± 0.13 | 3.61 ± 0.07 | 2.06 ± 0.15 | – | – |
Fork speeds were determined from fully IdU (red) and/or CIdU (green) substituted molecules. The number of red (NR), green (NG), and red-green (NRG) substituted molecules obtained for each segment is shown. The average speed per replication fork (kb/min) was calculated using the following equation: average kb/min = [length of segment (kb)/Td (min)]/average number of replication forks in the segment, where average number of replication forks in the segment = total forks/NRG molecules and Td (min) = the time required to duplicate a particular segment. Td (min) was determined using the following equation: Td (min) = Tp1 × NRG/(NR + NRG) or Td (min) = Tp2 × NRG/(NG + NRG) (Norio and Schildkraut, 2001), where Tp1 and Tp2 = length of time (240 min) of the red and green labeling pulses, respectively; NRG = the number of molecules fully stained in both red and green; and NR or NG = the number of molecules fully stained in red or green (Figs. S3, S4, and S5). Fork speeds and standard deviations were determined from averages of values obtained from NR and NG (n = 2–4). The duplication time for the terminal 100 kb of the telomeric segment (which included the telomere) was calculated using the following equation: Td = Tp1 × NRG100/(NR + NRG) or Td = Tp2 × NRG100/(NG + NRG) (Norio and Schildkraut, 2001, 2004), where NRG100 = the number of molecules fully stained in both red and green in the terminal 100 kb of the telomeric segment containing the telomere.