Table 1. Strains and plasmids used in... | Rockefeller University Press

Table 1.

Strains and plasmids used in this study

Strain name	Genotype	Source
AB33	a2 PnarbW2 PnarbE1, ble^R	Brachmann et al., 2001
EMD5 (AB33_GRab5a)	a2 PnarbW2 PnarbE1, ble^R/pCoGRab5a	This study
AB33ΔKin3	a2 PnarbW2 PnarbE1, ble^R, Δkin3, nat^R	Higuchi et al., 2014
AB33ΔKin3_GRab5a	a2 PnarbW2 PnarbE1, ble^R, Δkin3, nat^R/pCoGRab5a	Schuster et al., 2011b
AB5Dyn2^ts_GRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-dyn2ts, hyg^R/pCoGRab5a	Schuster et al., 2011b
AB33GRab5a	a2 PnarbW2 PnarbE1, ble^R/po^NGRab5a	Schuster et al., 2011a
AB33ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R	This study
AB33ΔHok1_Hok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/p^CHok1G	This study
EMD5_Hok1	a2 PnarbW2 PnarbE1, ble^R/pCoGRab5a/pHok1_SE	This study
AB33GRab5a_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po^NGRab5a	This study
AB33_mChRab5a_Hok1G	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R/po_mChRab5a	This study
AB33ΔHok1_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a	This study
AB33ΔHok1_mChRab5a_Hok1^625–930G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^625–930G	This study
AB33_mChTub1_Hok1^1–224G	a2 PnarbW2 PnarbE1, ble^R/pHo_mChTub1/pHok1^1–224G	This study
AB33ΔHok1_mChRab5a_Hok1^225–930G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^225–930G	This study
AB33ΔHok1_mChRab5a_Hok1^1–224G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–224G	This study
AB33ΔHok1_mChRab5a_Hok1^225–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^225–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–624PXG	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–624PXG	This study
AB33G₃Dyn2	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R	This study
AB33G₃Dyn2_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R, Δhok1, hyg^R	This study
AB33G₃Dyn2_ΔHok1_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R, Δhok1, hyg^R/po^C_mChRab5a	This study
AB33mCh₃Dyn2_ΔHok1_Hok1^1–624G	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3mcherry-dyn2, nat^R, Δhok1, hyg^R/pHok1^1–624G	This study
AB33ΔHok1_mChRab5a_Hok1^Δ225–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ225–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–224PXG	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–224PXG	This study
AB33_mChRab5a_Hok1G_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R/po_mChRab5a/pHk^361–385	This study
AB33ΔHok1_mChRab5a_Hok1^Δ361–385G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ361–385G	This study
AB33ΔHok1_mChRab5a_Hok1^Δ333–355G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ333–355G	This study
AB33GRab5a_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R/po^NGRab5a/pHk^361–385	This study
AB33Kin3G	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R	Schuster et al., 2011b
AB33Kin3G_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R, Δhok1, nat^R	This study
AB33Kin3G_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R/pHk^361–385	This study
AB33Kin3G_ΔHok1_Hok1^Δ361–385	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R, Δhok1, nat^R/pHok1^Δ361–385HA	This study
AB33G₃Dyn2_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R/po_mChRab5a	Schuster et al., 2011c
AB33ΔHok1_mChRab5a_pHok1^H3_1–600G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_1–600G	This study
AB33ΔHok1_mChRab5a_pHok1^H3_166–436G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_166–436G	This study
AB33ΔHok1_mChRab5a_pHok1^H3_293–345G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_293–345G	This study
AB33_mChRab5a_Fts1G	a2 PnarbW2 PnarbE1, ble^R, Pfts1-fts1-egfp, hyg^R/po_mChRab5a	This study
AB33_mChRab5a_Fhp1G	a2 PnarbW2 PnarbE1, ble^R, Pfhp1-fhp1-egfp, hyg^R/po_mChRab5a	This study
AB33_mChRab5a_Hok1G_ΔKin3	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R, Δkin3, cbx^R/po_mChRab5a	This study
AB33GRab7	a2 PnarbW2 PnarbE1, ble^R/poGRab7	Higuchi et al., 2014
AB33ΔHok1_GRab7	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/poGRab7	This study
AB33nRFP	a2 PnarbW2 PnarbE1, ble^R/poNLS3RFP	Schuster et al., 2011a
FB2N107G	a2b2 Pnup107-nup107-egfp, ble^R	Steinberg et al., 2012
pCoGRab5a	Potef-egfp-rab5a, cbx^R	Higuchi et al., 2014
pΔKin3	Δkin3, nat^R	Schuster et al., 2011c
po^NGRab5a	Potef-egfp-rab5a, nat^R	Schuster et al., 2011a
p^HΔHok1	Δhok1, hyg^R	This study
pHok1_SE	Phok1-hok1, SE, hyg^R	This study
p^CHok1G	Phok1-hok1-egfp, cbx^R	This study
po_mChRab5a	Potef-mcherry-rab5a, nat^R	Schuster et al., 2012
pHok1G	Phok1-hok1-3egfp, hyg^R	This study
pHok1^625–930G	Phok1-hok1^625–930-egfp, cbx^R	This study
pHok1^225–930G	Phok1-hok1^225–930-egfp, cbx^R	This study
pHok1^1–224G	Phok1-hok1^1–224-egfp, cbx^R	This study
pHok1^225–624G	Phok1-hok1^225–624-egfp, cbx^R	This study
pHok1^1–624G	Phok1-hok1^1–624-egfp, cbx^R	This study
pHok1^1–624PXG	Phok1-hok1^1–624-yup1^4–148-egfp, cbx^R	This study
pHo_mChTub1	Potef-mCherry-tub1, hyg^R	Schuster et al., 2012
p^NG₃Dyn2	Pdyn2-3gfp-dyn2, nat^R	This study
po^C_mChRab5a	Potef-mcherry-rab5a, cbx^R	This study
pHok1^Δ225–624G	Phok1-hok1^Δ225–624-egfp, cbx^R	This study
pHok1^1–224PXG	Phok1-hok1^1–224-yup1^4–148-egfp, cbx^R	This study
pHk^361–385	Pcrg-hok1^361–385, cbx^R	This study
pHok1^Δ361–385G	Phok1-hok1^Δ361–385-egfp, cbx^R	This study
pHok1^Δ333–355G	Phok1-hok1^Δ333–355-egfp, cbx^R	This study
pHok1^Δ361–385HA	Phok1-hok1^Δ361–385-HA, cbx^R	This study
pHok1^H3_1–600G	Phok1-hshook3^1–600-hok1^625–930-egfp, cbx^R	This study
pHok1^H3_166–436G	Phok1-hok1^1–224-hshook3^166–436-hok1^625–930-egfp, cbx^R	This study
pHok1^H3_293–345G	Phok1-hok1^1–332-hshook3^293–345-hok1^386–930-egfp, cbx^R	This study
pKin3G_H	Pkin3-kin3-egfp, hyg^R	Schuster et al., 2011c
p^NΔHok1	Δhok1, nat^R	This study
pFts1G	Pfts1-fts1-egfp, hyg^R	This study
pFhp1G	Pfhp1-fhp1-egfp, hyg^R	This study
p^CΔKin3	Δkin3, cbx^R	This study
poGRab7	Potef-egfp-rab7, cbx^R	Higuchi et al., 2014
pN107G	Pnup107-nup107-egfp, ble^R	Theisen et al., 2008

Strain name	Genotype	Source
AB33	a2 PnarbW2 PnarbE1, ble^R	Brachmann et al., 2001
EMD5 (AB33_GRab5a)	a2 PnarbW2 PnarbE1, ble^R/pCoGRab5a	This study
AB33ΔKin3	a2 PnarbW2 PnarbE1, ble^R, Δkin3, nat^R	Higuchi et al., 2014
AB33ΔKin3_GRab5a	a2 PnarbW2 PnarbE1, ble^R, Δkin3, nat^R/pCoGRab5a	Schuster et al., 2011b
AB5Dyn2^ts_GRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-dyn2ts, hyg^R/pCoGRab5a	Schuster et al., 2011b
AB33GRab5a	a2 PnarbW2 PnarbE1, ble^R/po^NGRab5a	Schuster et al., 2011a
AB33ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R	This study
AB33ΔHok1_Hok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/p^CHok1G	This study
EMD5_Hok1	a2 PnarbW2 PnarbE1, ble^R/pCoGRab5a/pHok1_SE	This study
AB33GRab5a_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po^NGRab5a	This study
AB33_mChRab5a_Hok1G	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R/po_mChRab5a	This study
AB33ΔHok1_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a	This study
AB33ΔHok1_mChRab5a_Hok1^625–930G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^625–930G	This study
AB33_mChTub1_Hok1^1–224G	a2 PnarbW2 PnarbE1, ble^R/pHo_mChTub1/pHok1^1–224G	This study
AB33ΔHok1_mChRab5a_Hok1^225–930G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^225–930G	This study
AB33ΔHok1_mChRab5a_Hok1^1–224G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–224G	This study
AB33ΔHok1_mChRab5a_Hok1^225–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^225–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–624PXG	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–624PXG	This study
AB33G₃Dyn2	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R	This study
AB33G₃Dyn2_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R, Δhok1, hyg^R	This study
AB33G₃Dyn2_ΔHok1_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R, Δhok1, hyg^R/po^C_mChRab5a	This study
AB33mCh₃Dyn2_ΔHok1_Hok1^1–624G	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3mcherry-dyn2, nat^R, Δhok1, hyg^R/pHok1^1–624G	This study
AB33ΔHok1_mChRab5a_Hok1^Δ225–624G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ225–624G	This study
AB33ΔHok1_mChRab5a_Hok1^1–224PXG	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^1–224PXG	This study
AB33_mChRab5a_Hok1G_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R/po_mChRab5a/pHk^361–385	This study
AB33ΔHok1_mChRab5a_Hok1^Δ361–385G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ361–385G	This study
AB33ΔHok1_mChRab5a_Hok1^Δ333–355G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^Δ333–355G	This study
AB33GRab5a_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R/po^NGRab5a/pHk^361–385	This study
AB33Kin3G	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R	Schuster et al., 2011b
AB33Kin3G_ΔHok1	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R, Δhok1, nat^R	This study
AB33Kin3G_↑pHk^361–385	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R/pHk^361–385	This study
AB33Kin3G_ΔHok1_Hok1^Δ361–385	a2 PnarbW2 PnarbE1, ble^R, Pkin3-kin3-egfp, hyg^R, Δhok1, nat^R/pHok1^Δ361–385HA	This study
AB33G₃Dyn2_mChRab5a	a2 PnarbW2 PnarbE1, ble^R, Pdyn2-3gfp-dyn2, nat^R/po_mChRab5a	Schuster et al., 2011c
AB33ΔHok1_mChRab5a_pHok1^H3_1–600G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_1–600G	This study
AB33ΔHok1_mChRab5a_pHok1^H3_166–436G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_166–436G	This study
AB33ΔHok1_mChRab5a_pHok1^H3_293–345G	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/po_mChRab5a/pHok1^H3_293–345G	This study
AB33_mChRab5a_Fts1G	a2 PnarbW2 PnarbE1, ble^R, Pfts1-fts1-egfp, hyg^R/po_mChRab5a	This study
AB33_mChRab5a_Fhp1G	a2 PnarbW2 PnarbE1, ble^R, Pfhp1-fhp1-egfp, hyg^R/po_mChRab5a	This study
AB33_mChRab5a_Hok1G_ΔKin3	a2 PnarbW2 PnarbE1, ble^R, Phok1-hok1-egfp, hyg^R, Δkin3, cbx^R/po_mChRab5a	This study
AB33GRab7	a2 PnarbW2 PnarbE1, ble^R/poGRab7	Higuchi et al., 2014
AB33ΔHok1_GRab7	a2 PnarbW2 PnarbE1, ble^R, Δhok1, hyg^R/poGRab7	This study
AB33nRFP	a2 PnarbW2 PnarbE1, ble^R/poNLS3RFP	Schuster et al., 2011a
FB2N107G	a2b2 Pnup107-nup107-egfp, ble^R	Steinberg et al., 2012
pCoGRab5a	Potef-egfp-rab5a, cbx^R	Higuchi et al., 2014
pΔKin3	Δkin3, nat^R	Schuster et al., 2011c
po^NGRab5a	Potef-egfp-rab5a, nat^R	Schuster et al., 2011a
p^HΔHok1	Δhok1, hyg^R	This study
pHok1_SE	Phok1-hok1, SE, hyg^R	This study
p^CHok1G	Phok1-hok1-egfp, cbx^R	This study
po_mChRab5a	Potef-mcherry-rab5a, nat^R	Schuster et al., 2012
pHok1G	Phok1-hok1-3egfp, hyg^R	This study
pHok1^625–930G	Phok1-hok1^625–930-egfp, cbx^R	This study
pHok1^225–930G	Phok1-hok1^225–930-egfp, cbx^R	This study
pHok1^1–224G	Phok1-hok1^1–224-egfp, cbx^R	This study
pHok1^225–624G	Phok1-hok1^225–624-egfp, cbx^R	This study
pHok1^1–624G	Phok1-hok1^1–624-egfp, cbx^R	This study
pHok1^1–624PXG	Phok1-hok1^1–624-yup1^4–148-egfp, cbx^R	This study
pHo_mChTub1	Potef-mCherry-tub1, hyg^R	Schuster et al., 2012
p^NG₃Dyn2	Pdyn2-3gfp-dyn2, nat^R	This study
po^C_mChRab5a	Potef-mcherry-rab5a, cbx^R	This study
pHok1^Δ225–624G	Phok1-hok1^Δ225–624-egfp, cbx^R	This study
pHok1^1–224PXG	Phok1-hok1^1–224-yup1^4–148-egfp, cbx^R	This study
pHk^361–385	Pcrg-hok1^361–385, cbx^R	This study
pHok1^Δ361–385G	Phok1-hok1^Δ361–385-egfp, cbx^R	This study
pHok1^Δ333–355G	Phok1-hok1^Δ333–355-egfp, cbx^R	This study
pHok1^Δ361–385HA	Phok1-hok1^Δ361–385-HA, cbx^R	This study
pHok1^H3_1–600G	Phok1-hshook3^1–600-hok1^625–930-egfp, cbx^R	This study
pHok1^H3_166–436G	Phok1-hok1^1–224-hshook3^166–436-hok1^625–930-egfp, cbx^R	This study
pHok1^H3_293–345G	Phok1-hok1^1–332-hshook3^293–345-hok1^386–930-egfp, cbx^R	This study
pKin3G_H	Pkin3-kin3-egfp, hyg^R	Schuster et al., 2011c
p^NΔHok1	Δhok1, nat^R	This study
pFts1G	Pfts1-fts1-egfp, hyg^R	This study
pFhp1G	Pfhp1-fhp1-egfp, hyg^R	This study
p^CΔKin3	Δkin3, cbx^R	This study
poGRab7	Potef-egfp-rab7, cbx^R	Higuchi et al., 2014
pN107G	Pnup107-nup107-egfp, ble^R	Theisen et al., 2008

a and b, mating type loci; P, promoter; a hyphen indicates fusion; Δ, deletion; a slash indicates ectopically integrated; hyg^R, hygromycin resistance; ble^R, phleomycin resistance; nat^R, nourseothricin resistance; cbx^R, carboxin resistance; ts, temperature-sensitive allele; crg, conditional arabinose-induced promoter; otef, constitutive promoter; nar, conditional nitrate reductase promoter; E1 and W2, genes of the b mating type locus; SE, self-replicating plasmid; mCherry, monomeric Cherry; kin3, kinesin-3; dyn2, C-terminal half of the dynein heavy chain; tub1, tubulin α; nup107, nucleoporin; PX, PX domain from the putative endosomal t-SNARE Yup1; rab5a and rab7, small endosomal GTPases; HA, human influenza HA tag; hok1, Hook protein in U. maydis; hshook3, Hook3 from human; fts1, FTS homologue; fhp1, FHIP homologue.

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