| Names of spindle proteins . | . | Curls/Tx-MTs . | . | Curls/Tx-MTs . | Ratios: 1D - 2D . |
|---|---|---|---|---|---|
| Gene name (and description) . | 1D separation . | 2D separation . | |||
| MAP1B (MT-associated protein) | 20/3.9 | 53/20 | 5.1 - 2.6 | ||
| CKAP5 (XMAP215 MT-associated protein) | 7.0/3.9 | 39/53 | 1.8 - 0.74 | ||
| EML4 (like echinoderm MAP4) | 2.0/0 | 5.0/0 | X - X | ||
| MAP 4 (MT-associated protein) | 3.0/11.8 | 41/33 | 0.25 - 1.24 | ||
| CLIP1 (CAP-GLY domain linker protein) | 4.0/2.0 | 16.6/8.4 | 2.0 - 2.0 | ||
| CLASP1 (CLIP-associating protein 1) | 1.0/7.8 | 5.0/12.1 | 0.13 - 0.41 | ||
| KIF5B (Kinesin-1 heavy chain) | 12/7.8 | 55/29 | 1.5 - 1.9 | ||
| KIF11 (Kinesin-like protein) | 4.0/3.9 | 22/25.6 | 1.0 - 0.86 | ||
| KIF15 (Kinesin-like protein) | 2.0/0.0 | 10/1.5 | X - 6.7 | ||
| KIF20A (Kinesin-like protein) | 1.0/7.8 | 7.0/15.1 | 0.13 - 0.46 | ||
| KLC1 (Kinesin light chain 1) | 4.6/4.7 | 9.2/4.5 | 0.98 - 2.0 | ||
| KLC2 (Kinesin light chain 2) | 4.2/9.0 | 8.1/12.1 | 0.47 - 0.67 | ||
| DYNC1H1 (Dynein 1 heavy chain 1) | 0/0 | 2.0/44 | X - 0.04 | ||
| DYNC1I2 (Dynein 1 intermediate chain 2) | 1.0/3.9 | 1.0/3.0 | 0.26 - 0.33 | ||
| DCTN1 (Dynactin subunit) | 0/0 | 0/6.0 | X - 0 | ||
| ZW10 (Dynein associated at kinetochore) | 0/0 | 0/10.5 | X - 0 | ||
| STMN1 (Stathmin) | 0/0 | 14/3.0 | X - 4.7 | ||
| TPX2 (Targets a kinetochore kinesin) | 0/2.0 | 4.1/2 | 0 - 2.0 | ||
| RCC1 (RAN nucleotide exchange factor) | 1.0/0 | 11/3 | X - 3.7 | ||
| NuMA (Nuclear-mitotic apparatus protein) | 5.0/3.9 | 16/13.6 | 1.3 - 1.8 | ||
| CENP-F (Centromere protein F) | 9.0/5.9 | 42/34.7 | 1.53 - 1.2 | ||
| CENP-E (Centromere protein E, a kinesin) | 0/0 | 0/12.1 | X - 0 | ||
| NDC80 (Conserved kinetochore MT bind) | 0/0 | 1/6.0 | X - 0.17 | ||
| SPC24 (Part of the NDC80 complex) | 0/0 | 1/3.0 | X - 0.33 | ||
| SPC25 (Part of the NDC80 complex) | 0/0 | 1.0/3.0 | X - 0.33 | ||
| NUF2 (Part of the NDC80 complex) | 0/0 | 3.0/3.0 | X - 1.0 | ||
| SKA1 (Kinetochore, interacts with NDC80) | 1.0/0 | 2.0/1.5 | X - 1.3 | ||
| SKA3 (Kinetochore, interacts with NDC80) | 0/0 | 1.0/1.5 | X - 0.67 | ||
| BUB3 (Spindle assembly checkpoint protein) | 5.0/2.0 | 4.0/3.0 | 2.5 - 1.3 | ||
| BUB1 (Spindle assembly CP protein) | 0/0 | 0/1.5 | X - 0 | ||
| MAD2L1 (Spindle assembly CP protein) | 1.0/0 | 1.0/1.5 | X - 0.67 | ||
| CLTC (Clathrin heavy chain 1) | 11/2.0 | 50/19.6 | 5.5 - 2.6 |
| Names of spindle proteins . | . | Curls/Tx-MTs . | . | Curls/Tx-MTs . | Ratios: 1D - 2D . |
|---|---|---|---|---|---|
| Gene name (and description) . | 1D separation . | 2D separation . | |||
| MAP1B (MT-associated protein) | 20/3.9 | 53/20 | 5.1 - 2.6 | ||
| CKAP5 (XMAP215 MT-associated protein) | 7.0/3.9 | 39/53 | 1.8 - 0.74 | ||
| EML4 (like echinoderm MAP4) | 2.0/0 | 5.0/0 | X - X | ||
| MAP 4 (MT-associated protein) | 3.0/11.8 | 41/33 | 0.25 - 1.24 | ||
| CLIP1 (CAP-GLY domain linker protein) | 4.0/2.0 | 16.6/8.4 | 2.0 - 2.0 | ||
| CLASP1 (CLIP-associating protein 1) | 1.0/7.8 | 5.0/12.1 | 0.13 - 0.41 | ||
| KIF5B (Kinesin-1 heavy chain) | 12/7.8 | 55/29 | 1.5 - 1.9 | ||
| KIF11 (Kinesin-like protein) | 4.0/3.9 | 22/25.6 | 1.0 - 0.86 | ||
| KIF15 (Kinesin-like protein) | 2.0/0.0 | 10/1.5 | X - 6.7 | ||
| KIF20A (Kinesin-like protein) | 1.0/7.8 | 7.0/15.1 | 0.13 - 0.46 | ||
| KLC1 (Kinesin light chain 1) | 4.6/4.7 | 9.2/4.5 | 0.98 - 2.0 | ||
| KLC2 (Kinesin light chain 2) | 4.2/9.0 | 8.1/12.1 | 0.47 - 0.67 | ||
| DYNC1H1 (Dynein 1 heavy chain 1) | 0/0 | 2.0/44 | X - 0.04 | ||
| DYNC1I2 (Dynein 1 intermediate chain 2) | 1.0/3.9 | 1.0/3.0 | 0.26 - 0.33 | ||
| DCTN1 (Dynactin subunit) | 0/0 | 0/6.0 | X - 0 | ||
| ZW10 (Dynein associated at kinetochore) | 0/0 | 0/10.5 | X - 0 | ||
| STMN1 (Stathmin) | 0/0 | 14/3.0 | X - 4.7 | ||
| TPX2 (Targets a kinetochore kinesin) | 0/2.0 | 4.1/2 | 0 - 2.0 | ||
| RCC1 (RAN nucleotide exchange factor) | 1.0/0 | 11/3 | X - 3.7 | ||
| NuMA (Nuclear-mitotic apparatus protein) | 5.0/3.9 | 16/13.6 | 1.3 - 1.8 | ||
| CENP-F (Centromere protein F) | 9.0/5.9 | 42/34.7 | 1.53 - 1.2 | ||
| CENP-E (Centromere protein E, a kinesin) | 0/0 | 0/12.1 | X - 0 | ||
| NDC80 (Conserved kinetochore MT bind) | 0/0 | 1/6.0 | X - 0.17 | ||
| SPC24 (Part of the NDC80 complex) | 0/0 | 1/3.0 | X - 0.33 | ||
| SPC25 (Part of the NDC80 complex) | 0/0 | 1.0/3.0 | X - 0.33 | ||
| NUF2 (Part of the NDC80 complex) | 0/0 | 3.0/3.0 | X - 1.0 | ||
| SKA1 (Kinetochore, interacts with NDC80) | 1.0/0 | 2.0/1.5 | X - 1.3 | ||
| SKA3 (Kinetochore, interacts with NDC80) | 0/0 | 1.0/1.5 | X - 0.67 | ||
| BUB3 (Spindle assembly checkpoint protein) | 5.0/2.0 | 4.0/3.0 | 2.5 - 1.3 | ||
| BUB1 (Spindle assembly CP protein) | 0/0 | 0/1.5 | X - 0 | ||
| MAD2L1 (Spindle assembly CP protein) | 1.0/0 | 1.0/1.5 | X - 0.67 | ||
| CLTC (Clathrin heavy chain 1) | 11/2.0 | 50/19.6 | 5.5 - 2.6 |
Gene names from NCBI, with descriptions in parentheses. For more information about each protein, see the same gene name in Tables S1 or S2. Spectral counts from 1D and 2D separations were normalized by the total number of counts for all proteins in that run and rounded to two significant figures, making the 1D and 2D counts more comparable. 2D numbers are generally more accurate because the initial number of counts was larger and farther above background. X implies no value. 0 implies not above noise. Differences in ratios from 1D and 2D data indicate that these numbers are only of semi-quantitative value.