Table 2.

Impact of SKAP55 mutants on SLP-76 microcluster dynamics in JSKAP.SY

SKAP55 chimera Persistence P Inward movement P Max speed P % SLP-76 in clusters P Clusters tracked per diameter P Total cells n (experiments) 
 s  µm  nm/s        
mRFP1 103.9 ± 35.2 †† 0.89 ± 0.13 †† 84.04 ± 15.6 †† 25.8 ± 6.8 †† 11.7 ± 1.2 † 18 7 
WT 235.3 ± 13.5 ** 3.27 ± 0.47 ** 131.1 ± 10.5 ** 67.3 ± 2.9 ** 15.4 ± 1 * 14 6 
Hom 221.2 ± 14.8 ** 3.29 ± 0.13 ** 188.4 ± 25.9 **, † 73.7 ± 0.4 ** 15.9 ± 0.57 * 10 3 
ΔDM 111.3 ± 23.8 †† 0.88 ± 0.09 †† 74.6 ± 18.4 †† 19.0 ± 7.2 †† 4.8 ± 0.92 **, †† 9 3 
TD 203.4 ± 20.9  2.95 ± 0.14 ** 129.5 ± 12.6  65.1 ± 2.8 ** 13.5 ± 1.3  10 3 
SKAP55 chimera Persistence P Inward movement P Max speed P % SLP-76 in clusters P Clusters tracked per diameter P Total cells n (experiments) 
 s  µm  nm/s        
mRFP1 103.9 ± 35.2 †† 0.89 ± 0.13 †† 84.04 ± 15.6 †† 25.8 ± 6.8 †† 11.7 ± 1.2 † 18 7 
WT 235.3 ± 13.5 ** 3.27 ± 0.47 ** 131.1 ± 10.5 ** 67.3 ± 2.9 ** 15.4 ± 1 * 14 6 
Hom 221.2 ± 14.8 ** 3.29 ± 0.13 ** 188.4 ± 25.9 **, † 73.7 ± 0.4 ** 15.9 ± 0.57 * 10 3 
ΔDM 111.3 ± 23.8 †† 0.88 ± 0.09 †† 74.6 ± 18.4 †† 19.0 ± 7.2 †† 4.8 ± 0.92 **, †† 9 3 
TD 203.4 ± 20.9  2.95 ± 0.14 ** 129.5 ± 12.6  65.1 ± 2.8 ** 13.5 ± 1.3  10 3 

SLP-76 microcluster dynamics in JSKAP.SY cells were measured as in Table 1. From JSKAP.SY cells expressing mRFP1: *, P < 0.05; **, P < 0.01. From JSKAP.SY with SKAP55.WT.mRFP1 add-back: †, P < 0.05; ††, P < 0.01.

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