Table 1. Summary of FRET efficiencies... | Rockefeller University Press

Table 1.

Summary of FRET efficiencies and percentage of active, extended KIF17 measured under all conditions described

Expressions	Treatments	E_AV	P-value	Active pool	P-value
		%		%
mCh-KIF17-EmGFP	Untreated	6.8 ± 0.2	NA	25.9 ± 1.4	NA
mCh-KIF17-EmGFP	1 mM AMP-PNP	2.4 ± 0.9	<0.001^a	58.4 ± 4.8	<0.001^a
mCh-KIF17^G754E-EmGFP	Untreated	2.3 ± 0.6	<0.001^a	70.1 ± 4.0	<0.001^a
mCh-KIF17-EmGFP	33 µM NZ	7.0 ± 0.4	>0.5^a	25.9 ± 3.7	>0.5^a
mCh-KIF17-EmGFP	Cold + 33 µM NZ	9.7 ± 0.5	<0.001^a	15.2 ± 3.5	<0.001^a
mCh-KIF17-EmGFP	100 nM OA	7.8 ± 0.4	<0.05^a	15.9 ± 2.6	<0.005^a
mCh-KIF17-EmGFP	10 µM taxol	9.3 ± 0.6	<0.001^a	11.4 ± 2.5	<0.001^a
mCh-KIF17-EmGFP	20 µM BIM-1	8.5 ± 0.5	<0.001^a	14.6 ± 1.7	<0.001^a
mCh-KIF17-EmGFP + Myc-EB1	Untreated	5.4 ± 0.4	<0.001^a	40.2 ± 4.3	<0.001^a
mCh-KIF17-EmGFP + Myc-EB1	33 µM NZ	6.7 ± 0.5	<0.005 ^b	24.2 ± 3.9	<0.001^b
mCh-KIF17-EmGFP + Myc-EB1	Cold + 33 µM NZ	8.5 ± 0.6	<0.001^b	16.3 ± 1.8	<0.001^b

Expressions	Treatments	E_AV	P-value	Active pool	P-value
		%		%
mCh-KIF17-EmGFP	Untreated	6.8 ± 0.2	NA	25.9 ± 1.4	NA
mCh-KIF17-EmGFP	1 mM AMP-PNP	2.4 ± 0.9	<0.001^a	58.4 ± 4.8	<0.001^a
mCh-KIF17^G754E-EmGFP	Untreated	2.3 ± 0.6	<0.001^a	70.1 ± 4.0	<0.001^a
mCh-KIF17-EmGFP	33 µM NZ	7.0 ± 0.4	>0.5^a	25.9 ± 3.7	>0.5^a
mCh-KIF17-EmGFP	Cold + 33 µM NZ	9.7 ± 0.5	<0.001^a	15.2 ± 3.5	<0.001^a
mCh-KIF17-EmGFP	100 nM OA	7.8 ± 0.4	<0.05^a	15.9 ± 2.6	<0.005^a
mCh-KIF17-EmGFP	10 µM taxol	9.3 ± 0.6	<0.001^a	11.4 ± 2.5	<0.001^a
mCh-KIF17-EmGFP	20 µM BIM-1	8.5 ± 0.5	<0.001^a	14.6 ± 1.7	<0.001^a
mCh-KIF17-EmGFP + Myc-EB1	Untreated	5.4 ± 0.4	<0.001^a	40.2 ± 4.3	<0.001^a
mCh-KIF17-EmGFP + Myc-EB1	33 µM NZ	6.7 ± 0.5	<0.005 ^b	24.2 ± 3.9	<0.001^b
mCh-KIF17-EmGFP + Myc-EB1	Cold + 33 µM NZ	8.5 ± 0.6	<0.001^b	16.3 ± 1.8	<0.001^b

NA, not applicable.

Value distribution as compared with mCh-KIF17-EmGFP using a two-tailed Student’s t test.

Value distribution as compared with mCh-KIF17-EmGFP + Myc-EB1 using a two-tailed Student’s t test.