Table 1.

Quantitation of cell shape in different KD and rescue conditions

Condition Maximum length Maximum width Perimeter 
 µm µm µm 
Control 33.09 ± 5.12 22.26 ± 3.35 88.44 ± 12.02 
WISp39 KD 91.69 ± 21.34a 19.58 ± 11.95a 220.26 ± 59.39a 
WISp39 siRNA + GFP-mWISp39WT 38.58 ± 8.76b 19.72 ± 4.87b 96.30 ± 21.53b 
WISp39 siRNA + GFP-mWISp39ΔTPR 85.13 ± 6.9a 18.09 ± 7.55a 265.96 ± 60.37a 
Coronin 1B KD 93.25 ± 11.36a 14.56 ± 5.79a 209.98 ± 25.22a 
p21 KD 36.60 ± 4.08b 23.56 ± 2.41b 96.18 ± 8.95b 
Condition Maximum length Maximum width Perimeter 
 µm µm µm 
Control 33.09 ± 5.12 22.26 ± 3.35 88.44 ± 12.02 
WISp39 KD 91.69 ± 21.34a 19.58 ± 11.95a 220.26 ± 59.39a 
WISp39 siRNA + GFP-mWISp39WT 38.58 ± 8.76b 19.72 ± 4.87b 96.30 ± 21.53b 
WISp39 siRNA + GFP-mWISp39ΔTPR 85.13 ± 6.9a 18.09 ± 7.55a 265.96 ± 60.37a 
Coronin 1B KD 93.25 ± 11.36a 14.56 ± 5.79a 209.98 ± 25.22a 
p21 KD 36.60 ± 4.08b 23.56 ± 2.41b 96.18 ± 8.95b 

Quantitation of cell size and shape parameters of the following cells: control, WISp39 KD, Coronin 1B KD, p21 KD, and WISp39 KD reconstituted with WT WISp39 or with WISp39ΔTPR.

a

P ≤ 0.001 compared to control cells; Student’s t test.

b

Not significant compared to control cells; Student’s t test.

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