Table 1.

Phosphorylated residues within the nonhelical tail domain of endogenous MHCII-B identified by MS

Residues Sequence Phosphoresidue Screening IMAC 
1930–1940 GGPISFSSSR 1935 + + 
1929–1940 RGGPISFSSSR 1935 + + 
1929–1940 RGGPISFSSSR 1937 − + 
1930–1940 GGPISFSSSR 1938 + + 
1929–1940 RGGPISFSSSR 1938 + + 
1930–1940 GGPISFSSSR 1939 + + 
1929–1940 RGGPISFSSSR 1939 + + 
1930–1940 GGPISFSSSR * − + 
1930–1940 RGGPISFSSSR * − + 
1958–1976 QLHLEGASLELSDDDTESK 1965 − + 
1958–1976 QLHLEGASLELSDDDTESK 1969 + + 
1958–1976 QLHLEGASLELSDDDTESK 1965 + 1969 + + 
Residues Sequence Phosphoresidue Screening IMAC 
1930–1940 GGPISFSSSR 1935 + + 
1929–1940 RGGPISFSSSR 1935 + + 
1929–1940 RGGPISFSSSR 1937 − + 
1930–1940 GGPISFSSSR 1938 + + 
1929–1940 RGGPISFSSSR 1938 + + 
1930–1940 GGPISFSSSR 1939 + + 
1929–1940 RGGPISFSSSR 1939 + + 
1930–1940 GGPISFSSSR * − + 
1930–1940 RGGPISFSSSR * − + 
1958–1976 QLHLEGASLELSDDDTESK 1965 − + 
1958–1976 QLHLEGASLELSDDDTESK 1969 + + 
1958–1976 QLHLEGASLELSDDDTESK 1965 + 1969 + + 

Asterisks indicate diphosphorylated forms of the peptide, although the specific combination of phosphorylated sites is not distinguishable from the MS/MS spectra. Plus signs denote positive detection of the indicated phosphorylation; minus signs indicates negative (no) detection of the indicated phosphorylation. Bold letters indicate the phosphorylated residue that has been positively detected or not detected within each peptide.

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