Primers used in this study
| Primers | Forward | Reverse |
| P[acman] FERM-GalK | 5′-TCATCCTCTTCATCATCATCTGCATTATCCGACGCAATCGGGGCGGAAAGCCTGTTGACAATTAATCATCGGCA-3′ | 5′-TCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGATCGTGGACATCTCAGCACTGTCCTGCTCCTT-3′ |
| P[acman] ΔFERM | 5′-TGGCCCTGGCCTTCATCATCATCCTCTTCATCATCATCTGCATTATCCGACGGGAGCTGGCCAACGGCCGGCGGGATTATCCCGAAGAGGGCGGATTCCA-3′ | 5′-TGGAATCCGCCCTCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGTCGGATAATGCAGATGATGATGAAGAGGATGATGATGAAGGCCAGGGCCA-3′ |
| pUAST-NrgS213L | 5′-CCGATTTCTACTATGCCTGCTTGGCCACCTCGGTGTTTCGCAG-3′ | 5′-CTGCGAAACACCGAGGTGGCCAAGCAGGCATAGTAGAAATCGG-3′ |
| Check and seq primers | ||
| P[acman] ΔFERM check | 5′-TCCATGTACAGGATCAAGG-3′ | 5′-ACTCTAACCTGTATCGCCATC-3′ |
| P[acman] ΔFERM seq | 5′-CTTTAACACGGAGAGTGCCAC-3′ | 5′-GATTTTGGGACTTACGGTTGC-3′ |
| Primers | Forward | Reverse |
| P[acman] FERM-GalK | 5′-TCATCCTCTTCATCATCATCTGCATTATCCGACGCAATCGGGGCGGAAAGCCTGTTGACAATTAATCATCGGCA-3′ | 5′-TCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGATCGTGGACATCTCAGCACTGTCCTGCTCCTT-3′ |
| P[acman] ΔFERM | 5′-TGGCCCTGGCCTTCATCATCATCCTCTTCATCATCATCTGCATTATCCGACGGGAGCTGGCCAACGGCCGGCGGGATTATCCCGAAGAGGGCGGATTCCA-3′ | 5′-TGGAATCCGCCCTCTTCGGGATAATCCCGCCGGCCGTTGGCCAGCTCCCGTCGGATAATGCAGATGATGATGAAGAGGATGATGATGAAGGCCAGGGCCA-3′ |
| pUAST-NrgS213L | 5′-CCGATTTCTACTATGCCTGCTTGGCCACCTCGGTGTTTCGCAG-3′ | 5′-CTGCGAAACACCGAGGTGGCCAAGCAGGCATAGTAGAAATCGG-3′ |
| Check and seq primers | ||
| P[acman] ΔFERM check | 5′-TCCATGTACAGGATCAAGG-3′ | 5′-ACTCTAACCTGTATCGCCATC-3′ |
| P[acman] ΔFERM seq | 5′-CTTTAACACGGAGAGTGCCAC-3′ | 5′-GATTTTGGGACTTACGGTTGC-3′ |