Table 1.

Quantitative information of the analyzed cells

Parameters Control (four cells) Augmin RNAi (four cells) 
Number of MTs (/10 µm3549 448 
Number of intraspindle MTs (/µm2)b 68a 32 
Number of closed ends (/10 µm3142 138 
Number of pole-facing open ends (/10 µm3) (flared, blunt) 66 (55, 11) 76 (64, 12) 
Number of ends near the centriole (/10 µm3) (closed, pole-facing open)c 186a (135, 51) 203 (135, 68) 
Number of ends away from the centriole (/10 µm3) (closed, pole-facing open)d 43a (23, 20) 10 (2, 8) 
Number of chromosome-facing open ends (/10 µm3) (flared, blunt) 83 (81, 2) 58 (52, 6) 
Percentage of end-links (per total closed ends) (rod shaped, direct) 8.8 (4.6, 4.2) 1.1 (0.6, 0.5) 
Number of end-links in total volume (rod shaped, direct) 71 in 57 µm3 (37, 34) 11 in 71 µm3 (6, 5) 
Number of end-links/10 µm3 (rod shaped, direct) 12 (6, 6) 1.5 (0.8, 0.7) 
Number of end-links/1-mm MT (rod shaped, direct) 27 (14, 13) 4.2 (2.3, 1.9) 
Percentage of end-links near the centriole (/closed ends) (rod shaped, direct)c 6.6a (3.1, 3.5) ND 
Percentage of end-links away from the centriole (/closed ends) (rod shaped, direct)d 16.4a (10, 6.4) ND 
Length of rod-shaped end-link (nm, ±SD) 29 ± 14 ND 
Angle α (°, ±SD); rod shaped 14 ± 40 ND 
Angle α (°, ±SD); direct 61 ± 47 ND 
Angle β (°, ±SD); rod shaped 64 ± 17 ND 
Angle γ (°, ±SD); rod shaped 131 ± 36 ND 
Parameters Control (four cells) Augmin RNAi (four cells) 
Number of MTs (/10 µm3549 448 
Number of intraspindle MTs (/µm2)b 68a 32 
Number of closed ends (/10 µm3142 138 
Number of pole-facing open ends (/10 µm3) (flared, blunt) 66 (55, 11) 76 (64, 12) 
Number of ends near the centriole (/10 µm3) (closed, pole-facing open)c 186a (135, 51) 203 (135, 68) 
Number of ends away from the centriole (/10 µm3) (closed, pole-facing open)d 43a (23, 20) 10 (2, 8) 
Number of chromosome-facing open ends (/10 µm3) (flared, blunt) 83 (81, 2) 58 (52, 6) 
Percentage of end-links (per total closed ends) (rod shaped, direct) 8.8 (4.6, 4.2) 1.1 (0.6, 0.5) 
Number of end-links in total volume (rod shaped, direct) 71 in 57 µm3 (37, 34) 11 in 71 µm3 (6, 5) 
Number of end-links/10 µm3 (rod shaped, direct) 12 (6, 6) 1.5 (0.8, 0.7) 
Number of end-links/1-mm MT (rod shaped, direct) 27 (14, 13) 4.2 (2.3, 1.9) 
Percentage of end-links near the centriole (/closed ends) (rod shaped, direct)c 6.6a (3.1, 3.5) ND 
Percentage of end-links away from the centriole (/closed ends) (rod shaped, direct)d 16.4a (10, 6.4) ND 
Length of rod-shaped end-link (nm, ±SD) 29 ± 14 ND 
Angle α (°, ±SD); rod shaped 14 ± 40 ND 
Angle α (°, ±SD); direct 61 ± 47 ND 
Angle β (°, ±SD); rod shaped 64 ± 17 ND 
Angle γ (°, ±SD); rod shaped 131 ± 36 ND 
a

Based on three cells for which the centriole center was detected in the tomographic volumes.

b

Numbers of MTs that pass through the plane 2.5 µm apart from the centriole center.

c

<1.5 µm from the centriole center.

d

≥1.5 µm from the centriole center.

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