Comparison of state-to-state frequency and growth/shrinkage rate between control and MoesinT559D- and MoesinK212,213M,T559D-expressing cells
| State to state and rates | Control | MoesinT559D | MoesinK211,212M,T559D |
| G → S | |||
| (ns−1) | 0.0114 (44) | 0.0055 (13) | 0.0125 (20) |
| (ns−1) | 0.0085–0.0148 | 0.0031–0.0091 | 0.0079–0.0184 |
| P = 0.0139a | P = 0.714 | ||
| G → P | |||
| (ns−1) | 0.0569 (220) | 0.062 (146) | 0.0579 (93) |
| (ns−1) | 0.0527–0.0609 | 0.0569–0.0665 | 0.0525–0.0635 |
| P = 0.134 | P = 0.783 | ||
| S → G | |||
| (ns−1) | 0.0110 (37) | 0.0142 (22) | 0.0062 (12) |
| (ns−1) | 0.0079–0.0147 | 0.0092–0.0203 | 0.0034–0.0104 |
| P = 0.298 | P = 0.0788 | ||
| S → P | |||
| (ns−1) | 0.0564 (190) | 0.0544 (84) | 0.0543 (105) |
| (ns−1) | 0.0521–0.0605 | 0.0481–0.0602 | 0.0483–0.0595 |
| P = 0.589 | P = 0.566 | ||
| P → G | |||
| (ns−1) | 0.0271 (148) | 0.0177 (85) | 0.0192 (54) |
| (ns−1) | 0.0229–0.0318 | 0.0139–0.0218 | 0.0143–0.0248 |
| P = 0.0047b | P = 0.037a | ||
| P → S | |||
| (ns−1) | 0.0216 (118) | 0.0121 (58) | 0.0214 (60) |
| (ns−1) | 0.018–0.0256 | 0.009–0.0158 | 0.0161–0.0275 |
| P = 0.0014c | P = 0.9401 | ||
| Pause state | |||
| (%) | 67.3 (3,421) | 78.2 (3,235) | 69.6 (1,863) |
| (%) | 66.0–68.6 | 76.9–79.4 | 67.8–71.3 |
| Shrinkage state | |||
| (%) | 14.8 (752) | 8.3 (343) | 15.8 (422) |
| (%) | 13.8–15.8 | 7.5–9.2 | 14.4–17.2 |
| Growth state | |||
| (%) | 17.9 (912) | 13.5 (558) | 14.6 (391) |
| (%) | 16.9–19.0 | 12.5–14.6 | 13.3–16.0 |
| Growth rate | |||
| (µm/min) | 2.68 | 2.39 | 2.55 |
| (µm/min) | ±0.052 | ±0.063 | ±0.078 |
| P = 4.3.10−4c | P=0.141 | ||
| Shrinkage rate | |||
| (µm/min) | 2.61 | 2.54 | 2.46 |
| (µm/min) | ±0.058 | ±0.09 | ±0.078 |
| P = 0.552 | P = 0.159 |
| State to state and rates | Control | MoesinT559D | MoesinK211,212M,T559D |
| G → S | |||
| (ns−1) | 0.0114 (44) | 0.0055 (13) | 0.0125 (20) |
| (ns−1) | 0.0085–0.0148 | 0.0031–0.0091 | 0.0079–0.0184 |
| P = 0.0139a | P = 0.714 | ||
| G → P | |||
| (ns−1) | 0.0569 (220) | 0.062 (146) | 0.0579 (93) |
| (ns−1) | 0.0527–0.0609 | 0.0569–0.0665 | 0.0525–0.0635 |
| P = 0.134 | P = 0.783 | ||
| S → G | |||
| (ns−1) | 0.0110 (37) | 0.0142 (22) | 0.0062 (12) |
| (ns−1) | 0.0079–0.0147 | 0.0092–0.0203 | 0.0034–0.0104 |
| P = 0.298 | P = 0.0788 | ||
| S → P | |||
| (ns−1) | 0.0564 (190) | 0.0544 (84) | 0.0543 (105) |
| (ns−1) | 0.0521–0.0605 | 0.0481–0.0602 | 0.0483–0.0595 |
| P = 0.589 | P = 0.566 | ||
| P → G | |||
| (ns−1) | 0.0271 (148) | 0.0177 (85) | 0.0192 (54) |
| (ns−1) | 0.0229–0.0318 | 0.0139–0.0218 | 0.0143–0.0248 |
| P = 0.0047b | P = 0.037a | ||
| P → S | |||
| (ns−1) | 0.0216 (118) | 0.0121 (58) | 0.0214 (60) |
| (ns−1) | 0.018–0.0256 | 0.009–0.0158 | 0.0161–0.0275 |
| P = 0.0014c | P = 0.9401 | ||
| Pause state | |||
| (%) | 67.3 (3,421) | 78.2 (3,235) | 69.6 (1,863) |
| (%) | 66.0–68.6 | 76.9–79.4 | 67.8–71.3 |
| Shrinkage state | |||
| (%) | 14.8 (752) | 8.3 (343) | 15.8 (422) |
| (%) | 13.8–15.8 | 7.5–9.2 | 14.4–17.2 |
| Growth state | |||
| (%) | 17.9 (912) | 13.5 (558) | 14.6 (391) |
| (%) | 16.9–19.0 | 12.5–14.6 | 13.3–16.0 |
| Growth rate | |||
| (µm/min) | 2.68 | 2.39 | 2.55 |
| (µm/min) | ±0.052 | ±0.063 | ±0.078 |
| P = 4.3.10−4c | P=0.141 | ||
| Shrinkage rate | |||
| (µm/min) | 2.61 | 2.54 | 2.46 |
| (µm/min) | ±0.058 | ±0.09 | ±0.078 |
| P = 0.552 | P = 0.159 |
Bold numbers denote statistically significant different values from controls. Numbers in parentheses indicate the event count. Intervals indicate 95% confidence. Statistical significance for sate-to-state transition frequencies was determined using a two-tailed permutation test with 10,000 resamples. For growth and shrinkage rates, a two-sample t test was used to determine statistical significance (±SEM).
Statistically different from control if P < 0.05.
P < 0.01.
P < 0.001.