Relevant parameters for ATM activation upon a subset of genotoxic treatments
| Parameter | Approach | Figure | NT | MMC (200 nM) | DOX (50 nM) | UV (5 J/m2) | CPT (25 nM) | CPT (1 µM) |
| Fork reversal | EM analysis | 3, B and C | −/+ | ++ | ++ | ++ | ++ | ++ |
| DSBs | PFGE | 1 B and S4 | − | − | −/+ | − | − | ++ |
| ATM signaling at forks | iPOND pRPA32 | 6 B | − | − | − | − | − | ++ |
| ATM signaling total | WB pRPA32 | 4 and S4 | − | − | − | − | − | ++ |
| ATM signaling total | WB pKAP1 | 4 and S4 | − | − | +/− | + | +/− | ++ |
| ATM signaling total | WB pATM | S4 | − | − | +/− | + | + | ++ |
| Parameter | Approach | Figure | NT | MMC (200 nM) | DOX (50 nM) | UV (5 J/m2) | CPT (25 nM) | CPT (1 µM) |
| Fork reversal | EM analysis | 3, B and C | −/+ | ++ | ++ | ++ | ++ | ++ |
| DSBs | PFGE | 1 B and S4 | − | − | −/+ | − | − | ++ |
| ATM signaling at forks | iPOND pRPA32 | 6 B | − | − | − | − | − | ++ |
| ATM signaling total | WB pRPA32 | 4 and S4 | − | − | − | − | − | ++ |
| ATM signaling total | WB pKAP1 | 4 and S4 | − | − | +/− | + | +/− | ++ |
| ATM signaling total | WB pATM | S4 | − | − | +/− | + | + | ++ |
Parameters were assessed by different investigation methods, as displayed in the indicated figures. −/+, +, ++, and +++ indicate increasingly clear phenotypes. NT, not treated; WB, Western blot.