Stiffness of matrices as determined by AFM
| Matrix | Elastic modulusc | SD | Na | nb |
| kPa | kPa | |||
| HDFC, nontreated | 4 | 1 | 4 | 685 |
| HDFC, cross-linked | 56 | 29 | 8 | 1,474 |
| Gelatin | 236 | 121 | 8 | 803 |
| 5% Gelatin cushions | 30 | 4 | 2 | 283 |
| Globular collagen | 216 | 24 | 2 | 345 |
| 3D collagen, 1.3 mg/ml | 1.7 | 2.6 | 4 | 893 |
| 3D collagen, 8.6 mg/ml | 6.3 | 2.3 | 7 | 1,785 |
| 3D collagen, 15 mg/ml | 17.7 | 9.8 | 10 | 2,402 |
| Matrix | Elastic modulusc | SD | Na | nb |
| kPa | kPa | |||
| HDFC, nontreated | 4 | 1 | 4 | 685 |
| HDFC, cross-linked | 56 | 29 | 8 | 1,474 |
| Gelatin | 236 | 121 | 8 | 803 |
| 5% Gelatin cushions | 30 | 4 | 2 | 283 |
| Globular collagen | 216 | 24 | 2 | 345 |
| 3D collagen, 1.3 mg/ml | 1.7 | 2.6 | 4 | 893 |
| 3D collagen, 8.6 mg/ml | 6.3 | 2.3 | 7 | 1,785 |
| 3D collagen, 15 mg/ml | 17.7 | 9.8 | 10 | 2,402 |
The elastic modulus is given as means ± SD. The raw AFM data used to derive mean and SD values of matrix stiffness are provided in dataset 1.
Number of independent sites measured in two to four independent experiments.
Number of independent points.
The differences between treatment groups (except for gelatin vs. globular collagen) were statistically significant according to Kruskal–Wallis test (P < 0.001) and Dunn’s post-test (P < 0.05).