Table 1. Oligonucleotide primers used in... | Rockefeller University Press

Table 1.

Oligonucleotide primers used in PCR reactions for cloning indicated genes into overexpression plasmids

Gene	Direction	Oligonucleotide primer sequence
shRNA-resistant K17^a	Forward	5′-GAGAATCTCAAGGAAGAACTAGCGTATCTGAAGAAGAACCACGAG-3′
	Reverse	5′-CTCGTGGTTCTTCTTCAGATACGCTAGTTCTTCCTTGAGATTCTC-3′
GFP-K17^b	Forward	5′-GACTGGATCCAGCATGGTGAGCAAGGGCGAGG-3′ (BamHI restriction site underlined)
	Reverse	5′-GCTGGGTCTAGATATCTCGAGTGTTAACGGGTGGTCTGGTGC-3′ (XbaI restriction site underlined)
GFP^c	Reverse	5′-GGTCTAGATTTACTTGTACAGCTCGTCCATGCCGAG-3′ (XbaI restriction site underlined)
hnRNP K^d	Forward	5′-CAGTCGACGATGGAAACTGAACAG-3′ (SalI restriction site underlined)
	Reverse	5′-ATCCCGGGCTTAGAATCCTTCAAC-3′ (XmaI restriction site underlined)

Gene	Direction	Oligonucleotide primer sequence
shRNA-resistant K17a	Forward	5′-GAGAATCTCAAGGAAGAACTAGCGTATCTGAAGAAGAACCACGAG-3′
	Reverse	5′-CTCGTGGTTCTTCTTCAGATACGCTAGTTCTTCCTTGAGATTCTC-3′
GFP-K17b	Forward	5′-GACTGGATCCAGCATGGTGAGCAAGGGCGAGG-3′ (BamHI restriction site underlined)
	Reverse	5′-GCTGGGTCTAGATATCTCGAGTGTTAACGGGTGGTCTGGTGC-3′ (XbaI restriction site underlined)
GFPc	Reverse	5′-GGTCTAGATTTACTTGTACAGCTCGTCCATGCCGAG-3′ (XbaI restriction site underlined)
hnRNP Kd	Forward	5′-CAGTCGACGATGGAAACTGAACAG-3′ (SalI restriction site underlined)
	Reverse	5′-ATCCCGGGCTTAGAATCCTTCAAC-3′ (XmaI restriction site underlined)

Used with a QuikChange II XL Site-Directed Mutagenesis kit.

Includes a BamHI or XbaI restriction site for ligation into pENTR1A plasmid (Invitrogen).

Used forward primer of GFP-K17^b for PCR reaction.

Includes a SalI or XmaI restriction site for ligation into mCherry-C1 plasmid (Takara Bio Inc.).