Quantification of comet tracking parameters
| Parameters | Telophase (cortical MTs) | ||
| Comet lifetime | Growth distance | Mean velocity | |
| s | µm | µm/s | |
| Control RNAi | |||
| EB1-GFP (359/21)a | 8.0 ± 3.7 | 2.9 ± 1.5 | 0.406 ± 0.12 |
| EB3 RNAi | |||
| EB1-GFP (467/30) | 11.0 ± 5.5c | 3.5 ± 1.9c | 0.349 ± 0.11c |
| EB3-FL (275/18) | 6.7 ± 2.4 | 2.2 ± 0.9 | 0.335 ± 0.11 |
| EB3-MT (276/16) | 6.3 ± 3.0 | 2.0 ± 1.1 | 0.318 ± 0.16 |
| EB3-S176A (282/14) | 6.6 ± 3.7 | 2.7 ± 1.7 | 0.407 ± 0.13b |
| EB3-S176D (197/12) | 12.2 ± 6.0b | 3.9 ± 1.9b | 0.330 ± 0.11 |
| Parameters | Telophase (cortical MTs) | ||
| Comet lifetime | Growth distance | Mean velocity | |
| s | µm | µm/s | |
| Control RNAi | |||
| EB1-GFP (359/21)a | 8.0 ± 3.7 | 2.9 ± 1.5 | 0.406 ± 0.12 |
| EB3 RNAi | |||
| EB1-GFP (467/30) | 11.0 ± 5.5c | 3.5 ± 1.9c | 0.349 ± 0.11c |
| EB3-FL (275/18) | 6.7 ± 2.4 | 2.2 ± 0.9 | 0.335 ± 0.11 |
| EB3-MT (276/16) | 6.3 ± 3.0 | 2.0 ± 1.1 | 0.318 ± 0.16 |
| EB3-S176A (282/14) | 6.6 ± 3.7 | 2.7 ± 1.7 | 0.407 ± 0.13b |
| EB3-S176D (197/12) | 12.2 ± 6.0b | 3.9 ± 1.9b | 0.330 ± 0.11 |
Numbers within parenthesis represent the number of tracked comets/number of cells in three independent experiments.
P < 0.001 when compared to EB3-FL (nonparametric ANOVA followed by a post-hoc Dunn’s test).
P < 0.001 when compared to EB1-GFP (nonparametric ANOVA followed by a post-hoc Dunn’s test).