Constructs used in the induced dimerization assay
| Construct | Construct designa | Accession number |
| FRB-KIF1A tail | FRB-3myc-YID-KIF1A391–1,698 | NM_008440 |
| FRB-KIF1Bβ tail | FRB-3myc-YKGGSGG-KIF1Bβ387–1,770 | NM_207682 |
| FRB-KIF13A tail | FRB-3myc-YKYSDLELKLRILQSTVPRA-KIF13A361–1,749 | NM_010617 |
| FRB-KIF13B442–1,826 (full length) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–1,826 | NM_001081177 |
| FRB-KIF13B442–831 (N-terminal fragment) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–831 | NM_001081177 |
| FRB-KIF13B832–1,826 (C-terminal fragment) tail | FRB-3myc-YKGGSGGSGG-KIF13B832–1,826 | NM_001081177 |
| GFP-BicD2-FKBP | GFP-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| tdTM-BicD2-FKBP | tdTM-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| BicD2-FKBP | FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| KIF5C-tdTM-FKBP | KIF5C1–559-ELGAPRPT-tdTM-FKBP | NM_001107730 |
| FRB-Rab5 | FRB-3myc-YKRTGSG-Rab5a | NM_025887 |
| FRB-Rab7 | FRB-3myc-YKRTGSG-Rab7 | NM_009005 |
| Construct | Construct designa | Accession number |
| FRB-KIF1A tail | FRB-3myc-YID-KIF1A391–1,698 | NM_008440 |
| FRB-KIF1Bβ tail | FRB-3myc-YKGGSGG-KIF1Bβ387–1,770 | NM_207682 |
| FRB-KIF13A tail | FRB-3myc-YKYSDLELKLRILQSTVPRA-KIF13A361–1,749 | NM_010617 |
| FRB-KIF13B442–1,826 (full length) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–1,826 | NM_001081177 |
| FRB-KIF13B442–831 (N-terminal fragment) tail | FRB-3myc-YKGGGSGGGSGGG-KIF13B442–831 | NM_001081177 |
| FRB-KIF13B832–1,826 (C-terminal fragment) tail | FRB-3myc-YKGGSGGSGG-KIF13B832–1,826 | NM_001081177 |
| GFP-BicD2-FKBP | GFP-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| tdTM-BicD2-FKBP | tdTM-MID-FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| BicD2-FKBP | FLAG-SH-BicD21–594-RTVSR-FKBP | AJ250106 |
| KIF5C-tdTM-FKBP | KIF5C1–559-ELGAPRPT-tdTM-FKBP | NM_001107730 |
| FRB-Rab5 | FRB-3myc-YKRTGSG-Rab5a | NM_025887 |
| FRB-Rab7 | FRB-3myc-YKRTGSG-Rab7 | NM_009005 |
Accession numbers were obtained from GenBank.
Includes epitope tag or label (FLAG, myc, GFP, or tdTM), heterodimerization domain (FRB or FKBP), amino acid linker sequence (using single letter code), and trafficking protein (amino acids included).