Table 1.

Constructs used in the induced dimerization assay

Construct Construct designa Accession number 
FRB-KIF1A tail FRB-3myc-YID-KIF1A391–1,698 NM_008440 
FRB-KIF1Bβ tail FRB-3myc-YKGGSGG-KIF1Bβ387–1,770 NM_207682 
FRB-KIF13A tail FRB-3myc-YKYSDLELKLRILQSTVPRA-KIF13A361–1,749 NM_010617 
FRB-KIF13B442–1,826 (full length) tail FRB-3myc-YKGGGSGGGSGGG-KIF13B442–1,826 NM_001081177 
FRB-KIF13B442–831 (N-terminal fragment) tail FRB-3myc-YKGGGSGGGSGGG-KIF13B442–831 NM_001081177 
FRB-KIF13B832–1,826 (C-terminal fragment) tail FRB-3myc-YKGGSGGSGG-KIF13B832–1,826 NM_001081177 
GFP-BicD2-FKBP GFP-MID-FLAG-SH-BicD21–594-RTVSR-FKBP AJ250106 
tdTM-BicD2-FKBP tdTM-MID-FLAG-SH-BicD21–594-RTVSR-FKBP AJ250106 
BicD2-FKBP FLAG-SH-BicD21–594-RTVSR-FKBP AJ250106 
KIF5C-tdTM-FKBP KIF5C1–559-ELGAPRPT-tdTM-FKBP NM_001107730 
FRB-Rab5 FRB-3myc-YKRTGSG-Rab5a NM_025887 
FRB-Rab7 FRB-3myc-YKRTGSG-Rab7 NM_009005 

Accession numbers were obtained from GenBank.

a

Includes epitope tag or label (FLAG, myc, GFP, or tdTM), heterodimerization domain (FRB or FKBP), amino acid linker sequence (using single letter code), and trafficking protein (amino acids included).

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