Extracellular proteins secreted in a ChiW-dependent manner identified by label-free mass spectrometry
| Identifier | Name | Sec signal peptide | Description | Intensity (relative intensitya) | Relative abundance Db10/ΔchiW | P-value | Unique peptides | Sequence coverage |
| % | ||||||||
| SMDB11_2875 | ChiB | No | Chitinase B | 8.5 × 109 (92) | 80.7 | 1.32 × 10−8 | 11 | 32 |
| SMDB11_0468 | ChiC | No | Chitinase C | 1.4 × 1010 (152) | 14.7 | 3.08 × 10−5 | 21 | 54 |
| SMDB11_3897 | − | No | Pirin | 1.3 × 108 (1.4) | 11.2 | 3.08 × 10−4 | 5 | 26 |
| SMDB11_4243 | ChiA | Yes | Chitinase A | 1.2 × 1011 (1,304) | 7.0 | 1.42 × 10−7 | 33 | 69 |
| SMDB11_1933 | TrpE | No | Anthranilate synthase | 5.5 × 108 (6) | 4.8 | 2.26 × 10−5 | 12 | 26 |
| SMDB11_4522 | MdhA | No | Malate dehydrogenase | 7.4 × 108 (8) | 3.4 | 1.94 × 10−5 | 9 | 36 |
| SMDB11_2877 | Cbp21 | Yes | Chitin binding protein | 6.7 × 1010 (728) | 3.4 | 0.0054 | 10 | 73 |
| SMDB11_2870 | MaeB | No | NADP-dependent malic enzyme | 1.3 × 108 (1.4) | 3.3 | 0.00032 | 11 | 18 |
| SMDB11_2390 | − | No | Putative heme oxygenase | 1.1 × 108 (1.2) | 3.1 | 0.0044 | 4 | 25 |
| SMDB11_3147 | ProS | No | Prolyl-tRNA synthetase | 9.2 × 107 (1) | 3.0 | 6.33 × 10−5 | 9 | 23 |
| Identifier | Name | Sec signal peptide | Description | Intensity (relative intensitya) | Relative abundance Db10/ΔchiW | P-value | Unique peptides | Sequence coverage |
| % | ||||||||
| SMDB11_2875 | ChiB | No | Chitinase B | 8.5 × 109 (92) | 80.7 | 1.32 × 10−8 | 11 | 32 |
| SMDB11_0468 | ChiC | No | Chitinase C | 1.4 × 1010 (152) | 14.7 | 3.08 × 10−5 | 21 | 54 |
| SMDB11_3897 | − | No | Pirin | 1.3 × 108 (1.4) | 11.2 | 3.08 × 10−4 | 5 | 26 |
| SMDB11_4243 | ChiA | Yes | Chitinase A | 1.2 × 1011 (1,304) | 7.0 | 1.42 × 10−7 | 33 | 69 |
| SMDB11_1933 | TrpE | No | Anthranilate synthase | 5.5 × 108 (6) | 4.8 | 2.26 × 10−5 | 12 | 26 |
| SMDB11_4522 | MdhA | No | Malate dehydrogenase | 7.4 × 108 (8) | 3.4 | 1.94 × 10−5 | 9 | 36 |
| SMDB11_2877 | Cbp21 | Yes | Chitin binding protein | 6.7 × 1010 (728) | 3.4 | 0.0054 | 10 | 73 |
| SMDB11_2870 | MaeB | No | NADP-dependent malic enzyme | 1.3 × 108 (1.4) | 3.3 | 0.00032 | 11 | 18 |
| SMDB11_2390 | − | No | Putative heme oxygenase | 1.1 × 108 (1.2) | 3.1 | 0.0044 | 4 | 25 |
| SMDB11_3147 | ProS | No | Prolyl-tRNA synthetase | 9.2 × 107 (1) | 3.0 | 6.33 × 10−5 | 9 | 23 |
Proteins identified as present in the secretome of the S. marcescens parental strain (Db10) at an abundance greater than 3× higher than observed in the secretome of a strain (JJH08p) lacking the chiW gene (P < 0.01) are shown. Four biological replicates of each strain were analyzed. The most abundant proteins in this list are highlighted in bold. Minus signs indicate that these gene products have no name.
Relative intensity is calculated in relation to the intensity of least abundant protein in the list (prolyl-tRNA synthetase). Additional mass spectrometry data showing the proteins in the secretome of the ΔchiW strain, which are increased in abundance relative to the secretome of the Db10 parental strain, are shown in Table S1.