Table 1. Identification of proteins by... | Rockefeller University Press

Table 1.

Identification of proteins by LC-MS/MS in co-IP experiments

Protein^a	Peptide coverage^b
	STT3B^c		STT3A^c	MagT1-V5^c
	cRM^d	HeLa^d	cRM^d	HeLa^d
STT3B	13	9	0	18
STT3A	0	0	3	0
Ribophorin I	75	42	47	39
Ribophorin II	58	37	13	44
OST48	53	44	43	40
MagT1	17	6	0	19
DAD1	35	19	19	19
Malectin	36	13	9	40
Calnexin^e	10	0	0	22
Calreticulin^e	0	0	0	34
Sec61α	0	0	0	0

Protein^a	Peptide coverage^b
	STT3B^c		STT3A^c	MagT1-V5^c
	cRM^d	HeLa^d	cRM^d	HeLa^d
STT3B	13	9	0	18
STT3A	0	0	3	0
Ribophorin I	75	42	47	39
Ribophorin II	58	37	13	44
OST48	53	44	43	40
MagT1	17	6	0	19
DAD1	35	19	19	19
Malectin	36	13	9	40
Calnexin^e	10	0	0	22
Calreticulin^e	0	0	0	34
Sec61α	0	0	0	0

OST subunits that were not detected in any sample include TUSC3, OST4, DC2, and KPC2. OST4, DC2, and KPC2 are low molecular weight hydrophobic membrane proteins, so they may not have been detected due to the limited number of tryptic fragments. TUSC3 is not expressed in HeLa cells.

Peptide coverage for the protein recognized by the precipitating antibody is low because the beads were eluted with a mixed-micelle immunoprecipitation wash buffer.

Antibody.

Membrane source. Digitonin solubilized HeLa cells or cRM were used for co-IP assays.

The presence of calnexin and calreticulin in co-IP samples could not be verified by protein immunoblotting.

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