Semiquantitative analysis of oskar mRNA and Oskar protein distribution in fixed oocytes raised at 18°C
| Genotype | Oocytes | oskar mRNA | Oskar protein | ||
| Off center | Width of PD | Localized fraction of mRNA | Width of PD | ||
| n | µm | µm | % | µm | |
| Stage 9 | |||||
| Wild type | 95 | 31.20 ± 1.72 | 9.49 ± 0.36 (1.0) | 56.9 ± 2.4 (1.0) | 10.64 ± 0.65 (1.0) |
| klarYG3/klarYG3 | 78 | 34.09 ± 1.60a | 9.11 ± 0.38 (0.650) | 67.2 ± 2.2 (0.002) | 12.02 ± 0.54a |
| Khc17/+; klarYG3/klarYG3 | 50; 25 for Oskar protein | 32.68 ± 2.25a | 10.57 ± 0.50 (0.026)b | 72.9 ± 2.1 (<0.001) | 11.73 ± 0.68a |
| klarYG3, oskA87/klarYG3, + | 67 | 32.28 ± 1.34a,b | 8.80 ± 0.32 (0.271)a | 71.6 ± 0.01 (<0.001) | 10.81 ± 0.0.51a |
| klarYG3/klar1 | 46 | 37.06 ± 1.91a,c | 9.64 ± 0.45 (0.193)c | 86.1 ± 4.0 (<0.001)c | 11.58 ± 0.56a,c |
| osk54/+ | 20 | 32.63 ± 2.59a | 9.94 ± 0.63 (0.228) | 73.0 ± 1.1 (0.002) | 12.44 ± 1.06a |
| Stage 10 | |||||
| Wild type | 87 | 65.57 ± 3.39 (1.0) | 12.43 ± 0.30 (1.0) | 60.7 ± 2.4 (1.0) | 18.58 ± 0.75 (1.0) |
| klarYG3/klarYG3 | 79 | 73.75 ± 2.19 (0.426) | 17.06 ± 0.69 (<0.001) | 66.0 ± 2.3 (0.115) | 20.24 ± 0.81 (0.146) |
| Khc17/+; klarYG3/klarYG3 | 62; 29 for Oskar protein | 80.68 ± 3.36 (<0.001)b | 15.21 ± 0.51 (<0.001) | 73.8 ± 1.5 (<0.001)b | 19.59 ± 1.04 (0.759) |
| klarYG3, oskA87/klarYG3, + | 63 | 73.49 ± 2.49 (0.137) | 13.07 ± 0.38b (0.162b) | 62.3 ± 1.8 (0.761) | 16.57 ± 0.64 (0.012)b |
| klarYG3/klar1 | 25 | 71.37 ± 2.46 (0.428)c | 22.52 ± 1.38 (<0.001)c | 79.5 ± 2.5 (0.001)c | 23.39 ± 1.54 (0.033)c |
| osk54/+ | 30 | 79.64 ± 2.39 (0.017) | 14.78 ± 0.65 (0.002) | 68.4 ± 1.9 (0.324) | 20.2 ± 0.94 (0.191) |
| Genotype | Oocytes | oskar mRNA | Oskar protein | ||
| Off center | Width of PD | Localized fraction of mRNA | Width of PD | ||
| n | µm | µm | % | µm | |
| Stage 9 | |||||
| Wild type | 95 | 31.20 ± 1.72 | 9.49 ± 0.36 (1.0) | 56.9 ± 2.4 (1.0) | 10.64 ± 0.65 (1.0) |
| klarYG3/klarYG3 | 78 | 34.09 ± 1.60a | 9.11 ± 0.38 (0.650) | 67.2 ± 2.2 (0.002) | 12.02 ± 0.54a |
| Khc17/+; klarYG3/klarYG3 | 50; 25 for Oskar protein | 32.68 ± 2.25a | 10.57 ± 0.50 (0.026)b | 72.9 ± 2.1 (<0.001) | 11.73 ± 0.68a |
| klarYG3, oskA87/klarYG3, + | 67 | 32.28 ± 1.34a,b | 8.80 ± 0.32 (0.271)a | 71.6 ± 0.01 (<0.001) | 10.81 ± 0.0.51a |
| klarYG3/klar1 | 46 | 37.06 ± 1.91a,c | 9.64 ± 0.45 (0.193)c | 86.1 ± 4.0 (<0.001)c | 11.58 ± 0.56a,c |
| osk54/+ | 20 | 32.63 ± 2.59a | 9.94 ± 0.63 (0.228) | 73.0 ± 1.1 (0.002) | 12.44 ± 1.06a |
| Stage 10 | |||||
| Wild type | 87 | 65.57 ± 3.39 (1.0) | 12.43 ± 0.30 (1.0) | 60.7 ± 2.4 (1.0) | 18.58 ± 0.75 (1.0) |
| klarYG3/klarYG3 | 79 | 73.75 ± 2.19 (0.426) | 17.06 ± 0.69 (<0.001) | 66.0 ± 2.3 (0.115) | 20.24 ± 0.81 (0.146) |
| Khc17/+; klarYG3/klarYG3 | 62; 29 for Oskar protein | 80.68 ± 3.36 (<0.001)b | 15.21 ± 0.51 (<0.001) | 73.8 ± 1.5 (<0.001)b | 19.59 ± 1.04 (0.759) |
| klarYG3, oskA87/klarYG3, + | 63 | 73.49 ± 2.49 (0.137) | 13.07 ± 0.38b (0.162b) | 62.3 ± 1.8 (0.761) | 16.57 ± 0.64 (0.012)b |
| klarYG3/klar1 | 25 | 71.37 ± 2.46 (0.428)c | 22.52 ± 1.38 (<0.001)c | 79.5 ± 2.5 (0.001)c | 23.39 ± 1.54 (0.033)c |
| osk54/+ | 30 | 79.64 ± 2.39 (0.017) | 14.78 ± 0.65 (0.002) | 68.4 ± 1.9 (0.324) | 20.2 ± 0.94 (0.191) |
Means ± SEM; numbers in parentheses indicate the p-value of a pairwise Mann–Whitney U test against the wild-type control of a given developmental stage performed after a Kruskal–Wallis test rejecting similarity of the data distributions (α = 0.05). Off center is the difference between the observed and the calculated center of mass along the AP axis. Width of PD is the onset of oskar mRNA or Oskar protein enrichment, when the observed signal uninterruptedly exceeds the double of the calculated or expected signal. Distance from the posterior pole. Localized fraction of mRNA shows the fraction of the oskar mRNA signal found in the posterior domain.
P-value of a Kruskal–Wallis test failing to reject similarity of the data distributions (α = 0.05).
Pairwise Mann–Whitney U test against the klarYG3/klarYG3 control with P < 0.05.
Pairwise comparison of klarYG3/klar1 with klarYG3/klarYG3 mutant oocytes using the appropriate tests.