Table 2. Semiquantitative analysis of... | Rockefeller University Press

Table 2.

Semiquantitative analysis of oskar mRNA and Oskar protein distribution in fixed oocytes raised at 25°C

Genotype	Oocytes	oskar mRNA			Oskar protein
		Off center	Width of PD	Localized fraction of mRNA	Width of PD
	n	µm	µm	%	µm
Stage 9
Wild type	70	26.41 ± 1.80 (1.0)	8.89 ± 0.36 (1.0)	51.2 ± 2.2 (1.0)	12.21 ± 0.58 (1.0)
klar^YG3/klar^YG3	33	31.73 ± 2.97 (0.13)	10.51 ± 0.76 (0.037)	62.3 ± 3.3 (0.007)	13.54 ± 0.83 (0.359)
Khc¹⁷/+; klar^YG3/klar^YG3	50	27.78 ± 2.14 (0.718)^a	9.98 ± 0.45 (0.05)^a	53.8 ± 2.8 (0.536)^a	10.34 ± 0.72 (0.012)^a
pat1^r/pat1^r; klar^YG3/klar^YG3	23	30.20 ± 2.33 (0.235)	9.26 ± 0.53 (0.401)^b	64.3 ± 2.9 (0.002)^b	9.36 ± 0.76 (0.004)^a^,^b
pat1^r/pat1^r	19	22.00 ± 4.513 (0.195)	18.93 ± 1.77 (<0.001)	47.1 ± 4.6 (0.614)	18.40 ± 2.86^c (0.015)
osk⁵⁴/+	33	32.83 ± 2.76 (0.06)	11.95 ± 0.64 (<0.001)	62.2 ± 2.9 (0.005)	12.53 ± 0.98 (0.343)
osk^A87/+	39	25.60 ± 1.79 (0.937)	9.50 ± 0.43 (0.061)	56.2 ± 2.5 (0.047)	9.669 ± 0.607 (0.002)
klar^YG3, osk^A87/klar^YG3, +	88	37.77 ± 1.31^a (<0.001)	9.70 ± 0.26 (0.032)	74.7 ± 0.8 (<0.001)^a	11.125 ± 0.441 (0.040)^a
klar^YG3/klar¹	32	31.30 ± 1.90 (0.870)^d	8.44 ± 0.55 (0.013)^d	77.5 ± 1.2 (<0.001)^d	ND
Stage 10
Wild type	62	56.05 ± 4.31 (1.0)	12.05 ± 0.45 (1.0)	51.7 ± 2.9 (1.0)	17.87 ± 0.61 (1.0)
klar^YG3/klar^YG3	42	68.57 ± 5.49 (0.026)	15.54 ± 0.61 (<0.001)	67.5 ± 3.1 (<0.001)	21.03 ± 0.80 (<0.001)
Khc¹⁷/+; klar^YG3/klar^YG3	59	64.24 ± 3.08 (0.440)^a	14.59 ± 0.40 (<0.001)	57.6 ± 2.7 (0.116)^a	17.85 ± 0.56 (0.809)^a
pat1^r/pat1^r; klar^YG3/klar^YG3	25	61.37 ± 2.91 (0.551)^a	13.03 ± 0.57 (0.214)^a^,^b	49.1 ± 3.9 (0.649)^a	17.45 ± 0.83 (0.855)^a^,^b
pat1^r/pat1^r	41	62.03 ± 4.915 (0.461)	16.64 ± 0.81 (<0.001)	46.9 ± 3.1 (0.306)	22.62 ± 0.96 (<0.001)
osk⁵⁴/+	35	73.38 ± 1.98 (0.033)	16.95 ± 0.66 (<0.001)	67.0 ± 2.5 (0.001)	18.25 ± 0.76 (0.496)
osk^A87/+	41	56.33 ± 3.38 (0.164)	12.30 ± 0.51 (0.654)	39.1 ± 2.3 (0.007)	16.26 ± 0.63 (0.166)
klar^YG3, osk^A87/klar^YG3, +	63	76.74 ± 2.89 (<0.001)	11.64 ± 0.35^a (0.647)	58.8 ± 2.3 (0.077)^a	15.86 ± 0.67 (0.112)^a
klar^YG3/klar¹	28	76.44 ± 4.16 (0.390)^d	13.85 ± 0.64 (0.094)^d	67.3 ± 3.1 (0.686)^d	ND

Genotype	Oocytes	oskar mRNA			Oskar protein
		Off center	Width of PD	Localized fraction of mRNA	Width of PD
	n	µm	µm	%	µm
Stage 9
Wild type	70	26.41 ± 1.80 (1.0)	8.89 ± 0.36 (1.0)	51.2 ± 2.2 (1.0)	12.21 ± 0.58 (1.0)
klar^YG3/klar^YG3	33	31.73 ± 2.97 (0.13)	10.51 ± 0.76 (0.037)	62.3 ± 3.3 (0.007)	13.54 ± 0.83 (0.359)
Khc¹⁷/+; klar^YG3/klar^YG3	50	27.78 ± 2.14 (0.718)^a	9.98 ± 0.45 (0.05)^a	53.8 ± 2.8 (0.536)^a	10.34 ± 0.72 (0.012)^a
pat1^r/pat1^r; klar^YG3/klar^YG3	23	30.20 ± 2.33 (0.235)	9.26 ± 0.53 (0.401)^b	64.3 ± 2.9 (0.002)^b	9.36 ± 0.76 (0.004)^a^,^b
pat1^r/pat1^r	19	22.00 ± 4.513 (0.195)	18.93 ± 1.77 (<0.001)	47.1 ± 4.6 (0.614)	18.40 ± 2.86^c (0.015)
osk⁵⁴/+	33	32.83 ± 2.76 (0.06)	11.95 ± 0.64 (<0.001)	62.2 ± 2.9 (0.005)	12.53 ± 0.98 (0.343)
osk^A87/+	39	25.60 ± 1.79 (0.937)	9.50 ± 0.43 (0.061)	56.2 ± 2.5 (0.047)	9.669 ± 0.607 (0.002)
klar^YG3, osk^A87/klar^YG3, +	88	37.77 ± 1.31^a (<0.001)	9.70 ± 0.26 (0.032)	74.7 ± 0.8 (<0.001)^a	11.125 ± 0.441 (0.040)^a
klar^YG3/klar¹	32	31.30 ± 1.90 (0.870)^d	8.44 ± 0.55 (0.013)^d	77.5 ± 1.2 (<0.001)^d	ND
Stage 10
Wild type	62	56.05 ± 4.31 (1.0)	12.05 ± 0.45 (1.0)	51.7 ± 2.9 (1.0)	17.87 ± 0.61 (1.0)
klar^YG3/klar^YG3	42	68.57 ± 5.49 (0.026)	15.54 ± 0.61 (<0.001)	67.5 ± 3.1 (<0.001)	21.03 ± 0.80 (<0.001)
Khc¹⁷/+; klar^YG3/klar^YG3	59	64.24 ± 3.08 (0.440)^a	14.59 ± 0.40 (<0.001)	57.6 ± 2.7 (0.116)^a	17.85 ± 0.56 (0.809)^a
pat1^r/pat1^r; klar^YG3/klar^YG3	25	61.37 ± 2.91 (0.551)^a	13.03 ± 0.57 (0.214)^a^,^b	49.1 ± 3.9 (0.649)^a	17.45 ± 0.83 (0.855)^a^,^b
pat1^r/pat1^r	41	62.03 ± 4.915 (0.461)	16.64 ± 0.81 (<0.001)	46.9 ± 3.1 (0.306)	22.62 ± 0.96 (<0.001)
osk⁵⁴/+	35	73.38 ± 1.98 (0.033)	16.95 ± 0.66 (<0.001)	67.0 ± 2.5 (0.001)	18.25 ± 0.76 (0.496)
osk^A87/+	41	56.33 ± 3.38 (0.164)	12.30 ± 0.51 (0.654)	39.1 ± 2.3 (0.007)	16.26 ± 0.63 (0.166)
klar^YG3, osk^A87/klar^YG3, +	63	76.74 ± 2.89 (<0.001)	11.64 ± 0.35^a (0.647)	58.8 ± 2.3 (0.077)^a	15.86 ± 0.67 (0.112)^a
klar^YG3/klar¹	28	76.44 ± 4.16 (0.390)^d	13.85 ± 0.64 (0.094)^d	67.3 ± 3.1 (0.686)^d	ND

Means ± SEM; the numbers in parentheses indicate the p-value of a pairwise Mann–Whitney U test against the wild-type control of a given developmental stage performed after a Kruskal–Wallis test rejecting similarity of the data distributions (α = 0.05). Off center represents the difference between the observed and the calculated center of mass along the AP axis. Width of PD is the onset of oskar mRNA or Oskar protein enrichment, when the observed signal uninterruptedly exceeds the double of the calculated or expected signal. Distance is measured from the posterior pole. Localized fraction of mRNA is the fraction of the oskar mRNA signal found in the posterior domain.

Pairwise Mann–Whitney U test against the klar^YG3/klar^YG3 control with P < 0.05.

Pairwise Mann–Whitney U test against the pat1^r/pat1^r control with P < 0.05.

Only 9 out of 19 oocytes had detectable Oskar protein.

Pairwise comparison of klar^YG3/klar¹ with klar^YG3/klar^YG3 mutant oocytes using the appropriate tests.

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