Fraction of motile RNPs are means ± SEM; the fraction of motile particles is statistically not different among the different genotypes at 25°C (Kruskal–Wallis test: P = 0.238; α = 0.05). Displacement and duration are means ± SD; SD is used to represent variance, as the data distribution is not greatly different from normal (Fig. 4 G); the numbers in parentheses indicate the p-value of a pairwise Mann–Whitney U test (α = 0.005) against the wild-type control after the equivalence of the different genotypes was rejected in a Kruskal–Wallis test (P < 0.001 for both displacement and duration). Velocity measurements are in means ± SEM; the numbers in parentheses indicate the p-value of a post hoc Dunnett’s T3 test (α = 0.005, equal variances are not assumed; Levene’s test: P < 0.001) against the wild type. The last column represents the resulting net velocity vector after vectorially averaging the velocities of oskar RNP runs in individual oocytes of a given genotype. None of the net velocity vectors are significantly different from 0 (two-tailed Wilcoxon sum ranks test: P > 0.05; α = 0.05) with the exception of pat1^r/pat1^r;;klar^YG3/klar^YG3.

Significant difference of motile fraction to wild-type control at 18°C (Mann–Whitney U test: α = 0.05; P < 0.05).

Significant difference of the motile fraction to klar^YG3 at 18°C (Mann–Whitney U test: α = 0.05; P < 0.05).

Significant difference between statistics of oskar RNP motility in the presence and absence of mutations impairing kinesin activity (Khc¹⁷ and pat1^r) in klar^YG3 homozygous oocytes; P < 0.005 and α = 0.005 for all statistics using the appropriate statistical probe.

Pairwise comparison of klar^YG3/klar¹ with klar^YG3/klar^YG3 mutant oocytes using the appropriate tests.

Statistically different value from 0.