Table 2.

List of general model parameters

Symbol Model parameter Value Reference 
Parameters used in both models    
Lkin Kinetochore linear size 430 nm Estimated based on McDonald et al., 1992  
katt Rate of binding events between new MTs and the kinetochore 4 min−1 Materials and methods part 2 section “Choice of model parameters” 
Ntotal Total number of NDC80 complexes per kinetochore Nsites × N0 = 600 By definition 
Δt Time step in computational algorithm 1 ms Materials and methods 
Additional parameters in the model with repetitive sites    
Nsites Number of MT binding sites per kinetochore 50 Estimated based on McEwen et al., 1997  
N0 Number of NDC80 complexes per MT binding site 12 Johnston et al., 2010; Lawrimore et al., 2011; Aravamudhan et al., 2013  
Additional parameters in the lawn model    
LNDC80 Length of NDC80 complex 60 nm Ciferri et al., 2008  
Lmin Minimal distance between KMTs 35 nm McDonald et al., 1992  
Symbol Model parameter Value Reference 
Parameters used in both models    
Lkin Kinetochore linear size 430 nm Estimated based on McDonald et al., 1992  
katt Rate of binding events between new MTs and the kinetochore 4 min−1 Materials and methods part 2 section “Choice of model parameters” 
Ntotal Total number of NDC80 complexes per kinetochore Nsites × N0 = 600 By definition 
Δt Time step in computational algorithm 1 ms Materials and methods 
Additional parameters in the model with repetitive sites    
Nsites Number of MT binding sites per kinetochore 50 Estimated based on McEwen et al., 1997  
N0 Number of NDC80 complexes per MT binding site 12 Johnston et al., 2010; Lawrimore et al., 2011; Aravamudhan et al., 2013  
Additional parameters in the lawn model    
LNDC80 Length of NDC80 complex 60 nm Ciferri et al., 2008  
Lmin Minimal distance between KMTs 35 nm McDonald et al., 1992  

These values are based on published data for PtK1 cells (for details see Materials and methods). All calculations in this work were carried out using these parameter values unless stated otherwise.

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