Kinetic constants used for modeling NDC80–MT interactions in both models
| Symbol | Model parameter | Construct/model | Value | Reference |
| s−1 | ||||
| kon | MT association rate for NDC80 complex | Site model | 1.52 | Based on model calibration (Fig. S3 A) |
| Lawn model | 0.80 | |||
| koff | MT dissociation rate for NDC80 complex | noD | 2.40 | Based on in vitro measurements with purified NDC80 proteins |
| 1D | 4.17 | |||
| 2D | 7.00 | |||
| 3D | 8.56 | |||
| 4D | 10.66 | |||
| 9D | 46.30 | |||
| ω | Cooperativity parameter for NDC80 complexes | 2.5a | Based on in vitro measurements with purified NDC80 proteins (unpublished data) |
| Symbol | Model parameter | Construct/model | Value | Reference |
| s−1 | ||||
| kon | MT association rate for NDC80 complex | Site model | 1.52 | Based on model calibration (Fig. S3 A) |
| Lawn model | 0.80 | |||
| koff | MT dissociation rate for NDC80 complex | noD | 2.40 | Based on in vitro measurements with purified NDC80 proteins |
| 1D | 4.17 | |||
| 2D | 7.00 | |||
| 3D | 8.56 | |||
| 4D | 10.66 | |||
| 9D | 46.30 | |||
| ω | Cooperativity parameter for NDC80 complexes | 2.5a | Based on in vitro measurements with purified NDC80 proteins (unpublished data) |
No dimension is associated with this value.