Effect of adenoviruses and PKA-targeting drugs on the dispersal of membranous organelles
| Organelle marker | Virus | Drug | Number of cells | Number of cells showing organelle dispersal | Cells showing organelle dispersal |
| % | |||||
| LysoTracker | − | − | 31 | 5 | 16.1 |
| LysoTracker | Ad5 | − | 46 | 39 | 84.8 |
| LysoTracker | Ad5 | H-89 (5 µM) | 31 | 11 | 35.5 |
| LysoTracker | Ad5 | SB203580 (10 µM) | 20 | 16 | 80.0 |
| LysoTracker | Ad5 | Cyclic RGD (8.5 µM) | 15 | 6 | 40.0 |
| LysoTracker | − | Forskolin (10 µM) | 32 | 25 | 78.1 |
| LysoTracker | Ad3 | − | 57 | 41 | 71.9 |
| LysoTracker | Ad3 | H-89 (5 µM) | 32 | 5 | 15.6 |
| GFP-Rab7 | Ad5 | − | 18 | 12 | 66.7 |
| GFP-Rab7 | − | − | 7 | 1 | 14.3 |
| GFP-NPC1 | Ad5 | − | 6 | 5 | 83.3 |
| GFP-NPC1 | − | − | 4 | 0 | 0.0 |
| GFP-NAGT | Ad5 | − | 13 | 0 | 0.0 |
| GFP-NAGT | − | − | 5 | 0 | 0.0 |
| MitoTracker | Ad5 | − | 20 | 1 | 5.0 |
| MitoTracker | − | − | 4 | 0 | 0.0 |
| Organelle marker | Virus | Drug | Number of cells | Number of cells showing organelle dispersal | Cells showing organelle dispersal |
| % | |||||
| LysoTracker | − | − | 31 | 5 | 16.1 |
| LysoTracker | Ad5 | − | 46 | 39 | 84.8 |
| LysoTracker | Ad5 | H-89 (5 µM) | 31 | 11 | 35.5 |
| LysoTracker | Ad5 | SB203580 (10 µM) | 20 | 16 | 80.0 |
| LysoTracker | Ad5 | Cyclic RGD (8.5 µM) | 15 | 6 | 40.0 |
| LysoTracker | − | Forskolin (10 µM) | 32 | 25 | 78.1 |
| LysoTracker | Ad3 | − | 57 | 41 | 71.9 |
| LysoTracker | Ad3 | H-89 (5 µM) | 32 | 5 | 15.6 |
| GFP-Rab7 | Ad5 | − | 18 | 12 | 66.7 |
| GFP-Rab7 | − | − | 7 | 1 | 14.3 |
| GFP-NPC1 | Ad5 | − | 6 | 5 | 83.3 |
| GFP-NPC1 | − | − | 4 | 0 | 0.0 |
| GFP-NAGT | Ad5 | − | 13 | 0 | 0.0 |
| GFP-NAGT | − | − | 5 | 0 | 0.0 |
| MitoTracker | Ad5 | − | 20 | 1 | 5.0 |
| MitoTracker | − | − | 4 | 0 | 0.0 |
Dispersal was scored based on 90-min time-lapse videos. Minus signs indicate that either no virus or no drug was added.