Oligonucleotide primers used for quantitative real-time RT-PCR
| Gene | Primer sequence (strand) | Product size | Temperature | PCR cycles | Reference or accession no. |
| bp | °C | ||||
| E-cadherin (Cdh1) | 5′-CTGCGCTGGATAGTGTGTGT-3′ (+) | 187 | 58 | 40 | NM_009864 |
| 5′-TGGCATGCACCTAAGAATCA-3′ (−) | |||||
| Keratin-19 (Krt19) | 5′-GATCAGCGGTTTTGAAGCCC-3′ (+) | 195 | 58 | 40 | NM_008471 |
| 5′-GTCTCGCTGGTAGCTCAGAT-3′ (−) | |||||
| Mucin-1 (Muc1) | 5′-AGACCCCAGCTCCAACTACT-3′ (+) | 178 | 58 | 40 | NM_013605 |
| 5′-TGACTTCACGTCAGAGGCAC-3′ (−) | |||||
| N-cadherin (Cdh2) | 5′-GAGAGGCCTATCCATGCTGA-3′ (+) | 204 | 58 | 40 | NM_007664 |
| 5′-CGCTACTGGAGGAGTTGAGG-3′ (−) | |||||
| Vimentin (Vim) | 5′-GTGCGCCAGCAGTATGAAAG-3′ (+) | 110 | 58 | 40 | NM_011701 |
| 5′-GCATCGTTGTTCCGGTTGG-3′ (−) | |||||
| Fibronectin (Fn1) | 5′-CCCAGACTTATGGTGGCAATTC-3′ (+) | 200 | 58 | 40 | NM_010233 |
| 5′-AATTTCCGCCTCGAGTCTGA-3′ (−) | |||||
| Hmga2 | 5′-AGCAAAAACAAGAGCCCCTCTA-3′ (+) | 100 | 58 | 40 | Thuault et al., 2006 |
| 5′-ACGACTTGTTGTGGCCATTTC-3′ (−) | |||||
| Snail1 (Snai1) | 5′-CCACTGCAACCGTGCTTTT-3′ (+) | 66 | 58 | 40 | Thuault et al., 2008 |
| 5′-CACATCCGAGTGGGTTTGG-3′ (−) | |||||
| Zeb1 | 5′-ACAAGACACCGCCGTCATTT-3′ (+) | 120 | 58 | 40 | Thuault et al., 2008 |
| 5′-GCAGGTGAGCAACTGGGAAA-3′ (−) | |||||
| Gapdh | 5′-TGTGTCCGTCGTGGATCTGA-3′ (+) | 76 | 58 | 40 | Valcourt et al., 2007 |
| 5′-CCTGCTTCACCACCTTCTTGA-3′ (−) |
| Gene | Primer sequence (strand) | Product size | Temperature | PCR cycles | Reference or accession no. |
| bp | °C | ||||
| E-cadherin (Cdh1) | 5′-CTGCGCTGGATAGTGTGTGT-3′ (+) | 187 | 58 | 40 | NM_009864 |
| 5′-TGGCATGCACCTAAGAATCA-3′ (−) | |||||
| Keratin-19 (Krt19) | 5′-GATCAGCGGTTTTGAAGCCC-3′ (+) | 195 | 58 | 40 | NM_008471 |
| 5′-GTCTCGCTGGTAGCTCAGAT-3′ (−) | |||||
| Mucin-1 (Muc1) | 5′-AGACCCCAGCTCCAACTACT-3′ (+) | 178 | 58 | 40 | NM_013605 |
| 5′-TGACTTCACGTCAGAGGCAC-3′ (−) | |||||
| N-cadherin (Cdh2) | 5′-GAGAGGCCTATCCATGCTGA-3′ (+) | 204 | 58 | 40 | NM_007664 |
| 5′-CGCTACTGGAGGAGTTGAGG-3′ (−) | |||||
| Vimentin (Vim) | 5′-GTGCGCCAGCAGTATGAAAG-3′ (+) | 110 | 58 | 40 | NM_011701 |
| 5′-GCATCGTTGTTCCGGTTGG-3′ (−) | |||||
| Fibronectin (Fn1) | 5′-CCCAGACTTATGGTGGCAATTC-3′ (+) | 200 | 58 | 40 | NM_010233 |
| 5′-AATTTCCGCCTCGAGTCTGA-3′ (−) | |||||
| Hmga2 | 5′-AGCAAAAACAAGAGCCCCTCTA-3′ (+) | 100 | 58 | 40 | Thuault et al., 2006 |
| 5′-ACGACTTGTTGTGGCCATTTC-3′ (−) | |||||
| Snail1 (Snai1) | 5′-CCACTGCAACCGTGCTTTT-3′ (+) | 66 | 58 | 40 | Thuault et al., 2008 |
| 5′-CACATCCGAGTGGGTTTGG-3′ (−) | |||||
| Zeb1 | 5′-ACAAGACACCGCCGTCATTT-3′ (+) | 120 | 58 | 40 | Thuault et al., 2008 |
| 5′-GCAGGTGAGCAACTGGGAAA-3′ (−) | |||||
| Gapdh | 5′-TGTGTCCGTCGTGGATCTGA-3′ (+) | 76 | 58 | 40 | Valcourt et al., 2007 |
| 5′-CCTGCTTCACCACCTTCTTGA-3′ (−) |
Accession numbers were obtained from GenBank.