Table 1.

Flagellar proteins with changing abundance identified by 2D-DIGE analysis

 21°C only 21 and 34°C 34°C only 
dhc1b-3 depletion vs. wt Class D1 (mut-34A): eEF1A1, CAH6, IFT80, Rib43a, IFT81, v4 315103, IFT27, RSP9, BUG10 (FAP252), FAP144, FLA8 or KLP1 Class D2 (mut-34NC): FAP103, FAP162, FAP102, FAP147, RSP10, v4 145209, RAN1 Class D3 (mut-34D): FAP103, CAM1 (calmodulin), FAP55, FAP127, FAP166, FAP189 or FAP58, KLP1, PP2C1c 
dhc1b-3 accumulation vs. wt Class A1 (mut-34D): FAP12, FAP215, ODA6 (ODA-IC2), FAP43, FAP31 or FAP197, PGM1b or ODA1, FAP203, BUG13 (FAP90), EB1, FAP57, IDA4 (p28), FAP18, BUG10 (FAP252) Class A2 (mut-34NC): CAH6, KLP1, ODA1 (ODA-DC2), FAP7, FAP219, RSP3, AGG3, FAP172, FAK1 or FAP66, FAP115, ODA9 (ODA-IC1), PF2 (DRC4), BUG10 (FAP252), Rib72 Class A3 (mut-34A): IFT121, IFT122, CAH6, FAP59, ODA9 (ODA-IC1), RSP16, FAP191 or AGG3 
No change RSP10, Rib43a, FAP164, ICL1, ODA13 (ODA-LC6), IDA5 (actin), IFT81, FAP161, IFT88, FAP52 
 21°C only 21 and 34°C 34°C only 
dhc1b-3 depletion vs. wt Class D1 (mut-34A): eEF1A1, CAH6, IFT80, Rib43a, IFT81, v4 315103, IFT27, RSP9, BUG10 (FAP252), FAP144, FLA8 or KLP1 Class D2 (mut-34NC): FAP103, FAP162, FAP102, FAP147, RSP10, v4 145209, RAN1 Class D3 (mut-34D): FAP103, CAM1 (calmodulin), FAP55, FAP127, FAP166, FAP189 or FAP58, KLP1, PP2C1c 
dhc1b-3 accumulation vs. wt Class A1 (mut-34D): FAP12, FAP215, ODA6 (ODA-IC2), FAP43, FAP31 or FAP197, PGM1b or ODA1, FAP203, BUG13 (FAP90), EB1, FAP57, IDA4 (p28), FAP18, BUG10 (FAP252) Class A2 (mut-34NC): CAH6, KLP1, ODA1 (ODA-DC2), FAP7, FAP219, RSP3, AGG3, FAP172, FAK1 or FAP66, FAP115, ODA9 (ODA-IC1), PF2 (DRC4), BUG10 (FAP252), Rib72 Class A3 (mut-34A): IFT121, IFT122, CAH6, FAP59, ODA9 (ODA-IC1), RSP16, FAP191 or AGG3 
No change RSP10, Rib43a, FAP164, ICL1, ODA13 (ODA-LC6), IDA5 (actin), IFT81, FAP161, IFT88, FAP52 

Proteins are grouped by relative accumulation or depletion in dhc1b-3 flagella at 21 and 34°C vs. wild-type flagella at 21°C (as plotted by blue and red bars in Fig. 6). Duplicate hits, tubulin, and proteins not found in the flagellar proteome have been excluded from this list. For the complete catalogue and description of analyzed 2D-DIGE spots, see Fig. S5 and Table S3. Classes D1 and A3 are proteins accumulated in dhc1b-3 flagella after 18 h at 34°C vs. dhc1b-3 flagella at 21°C (as plotted by green bars in Fig. 6, indicated on the table by “mut-34A”). Classes D3 and A1 are proteins depleted in dhc1b-3 flagella after 18 h at 34°C vs. dhc1b-3 flagella at 21°C (indicated by “mut-34D”). Proteins in classes D2 and A2 experience no change in flagellar abundance when dhc1b-3 cells are shifted from 21 to 34°C (indicated by “mut-34NC”). Proteins are listed in descending order of fold accumulation or depletion (except the “No change” class).

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