Flagellar proteins with changing abundance identified by 2D-DIGE analysis
| 21°C only | 21 and 34°C | 34°C only | |
| dhc1b-3 depletion vs. wt | Class D1 (mut-34A): eEF1A1, CAH6, IFT80, Rib43a, IFT81, v4 315103, IFT27, RSP9, BUG10 (FAP252), FAP144, FLA8 or KLP1 | Class D2 (mut-34NC): FAP103, FAP162, FAP102, FAP147, RSP10, v4 145209, RAN1 | Class D3 (mut-34D): FAP103, CAM1 (calmodulin), FAP55, FAP127, FAP166, FAP189 or FAP58, KLP1, PP2C1c |
| dhc1b-3 accumulation vs. wt | Class A1 (mut-34D): FAP12, FAP215, ODA6 (ODA-IC2), FAP43, FAP31 or FAP197, PGM1b or ODA1, FAP203, BUG13 (FAP90), EB1, FAP57, IDA4 (p28), FAP18, BUG10 (FAP252) | Class A2 (mut-34NC): CAH6, KLP1, ODA1 (ODA-DC2), FAP7, FAP219, RSP3, AGG3, FAP172, FAK1 or FAP66, FAP115, ODA9 (ODA-IC1), PF2 (DRC4), BUG10 (FAP252), Rib72 | Class A3 (mut-34A): IFT121, IFT122, CAH6, FAP59, ODA9 (ODA-IC1), RSP16, FAP191 or AGG3 |
| No change | RSP10, Rib43a, FAP164, ICL1, ODA13 (ODA-LC6), IDA5 (actin), IFT81, FAP161, IFT88, FAP52 | ||
| 21°C only | 21 and 34°C | 34°C only | |
| dhc1b-3 depletion vs. wt | Class D1 (mut-34A): eEF1A1, CAH6, IFT80, Rib43a, IFT81, v4 315103, IFT27, RSP9, BUG10 (FAP252), FAP144, FLA8 or KLP1 | Class D2 (mut-34NC): FAP103, FAP162, FAP102, FAP147, RSP10, v4 145209, RAN1 | Class D3 (mut-34D): FAP103, CAM1 (calmodulin), FAP55, FAP127, FAP166, FAP189 or FAP58, KLP1, PP2C1c |
| dhc1b-3 accumulation vs. wt | Class A1 (mut-34D): FAP12, FAP215, ODA6 (ODA-IC2), FAP43, FAP31 or FAP197, PGM1b or ODA1, FAP203, BUG13 (FAP90), EB1, FAP57, IDA4 (p28), FAP18, BUG10 (FAP252) | Class A2 (mut-34NC): CAH6, KLP1, ODA1 (ODA-DC2), FAP7, FAP219, RSP3, AGG3, FAP172, FAK1 or FAP66, FAP115, ODA9 (ODA-IC1), PF2 (DRC4), BUG10 (FAP252), Rib72 | Class A3 (mut-34A): IFT121, IFT122, CAH6, FAP59, ODA9 (ODA-IC1), RSP16, FAP191 or AGG3 |
| No change | RSP10, Rib43a, FAP164, ICL1, ODA13 (ODA-LC6), IDA5 (actin), IFT81, FAP161, IFT88, FAP52 | ||
Proteins are grouped by relative accumulation or depletion in dhc1b-3 flagella at 21 and 34°C vs. wild-type flagella at 21°C (as plotted by blue and red bars in Fig. 6). Duplicate hits, tubulin, and proteins not found in the flagellar proteome have been excluded from this list. For the complete catalogue and description of analyzed 2D-DIGE spots, see Fig. S5 and Table S3. Classes D1 and A3 are proteins accumulated in dhc1b-3 flagella after 18 h at 34°C vs. dhc1b-3 flagella at 21°C (as plotted by green bars in Fig. 6, indicated on the table by “mut-34A”). Classes D3 and A1 are proteins depleted in dhc1b-3 flagella after 18 h at 34°C vs. dhc1b-3 flagella at 21°C (indicated by “mut-34D”). Proteins in classes D2 and A2 experience no change in flagellar abundance when dhc1b-3 cells are shifted from 21 to 34°C (indicated by “mut-34NC”). Proteins are listed in descending order of fold accumulation or depletion (except the “No change” class).