Table 1.

Formation of subcutaneous tumors in nude mice by PGAM2-Tg MEFs infected with Ras-G12V and various Mdm2 mutants

Cells Number tumors/number injected 
PGAM2-Tg MEFs/Ras-G12V + vector 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-WT 0/12 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-Y281H 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-W329G 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-M459I 5/8 
Wild-type MEFs/Ras-G12V + E6 6/6 
Cells Number tumors/number injected 
PGAM2-Tg MEFs/Ras-G12V + vector 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-WT 0/12 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-Y281H 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-W329G 0/6 
PGAM2-Tg MEFs/Ras-G12V + Mdm2-M459I 5/8 
Wild-type MEFs/Ras-G12V + E6 6/6 

Formation of subcutaneous tumors in nude mice after injection of PGAM2-Tg MEFs infected with the indicated expression vectors. For each injection, 5 × 106 cells were injected in a volume of 100 µl. Mice were sacrificed after 3 wk of monitoring. Representative results of two independent experiments are shown (n = 2).

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