Table 2.

POM152 deletion affects growth and binding of ndc1 mutants

 Binding by MYTHa Growth on 5-FOAb 
Genotype Pom34 Nbp1 Mps3  
NDC1 ++ ++ + ++ 
pom152Δ ++ ++ ++ ++ 
ndc1-39 ++ −/+ − ts 
pom152Δ ndc1-39 ++ − + SS 
ndc1-L562S ++ ++ −/+ − 
pom152Δ ndc1-L562S ++ ++ ++ ++ 

SS, synthetic sick.

a

++, indicates robust binding; +, indicates binding; −/+, indicates weak binding; −, indicates no binding.

b

++, indicates growth at all temperatures; −, indicates no growth at all temperatures; ts, indicates growth at 23°C but not 37°C.

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