Signaling components required for the formation of NEMO- and IRAK1-containing structures and NF-κB activation in response to IL-1
| Cell genotype | Cell type | NEMO-containing clusters | IRAK1-containing clusters | NF-κB activation (nuc. p65) |
| Control fibro. | Human fibroblast | +++ | +++ | +++ |
| NEMO−/− | Human fibroblast | − | +++ | − |
| MyD88−/− | Human fibroblast | − | − | − |
| IRAK1−/− | HEK-293 | − | − | − |
| IRAK1−/− compl. IRAK1 | HEK-293 | +++ | +++ | +++ |
| IRAK4−/− | Human fibroblast | +/− | +++ | +/− |
| Cell genotype | Cell type | NEMO-containing clusters | IRAK1-containing clusters | NF-κB activation (nuc. p65) |
| Control fibro. | Human fibroblast | +++ | +++ | +++ |
| NEMO−/− | Human fibroblast | − | +++ | − |
| MyD88−/− | Human fibroblast | − | − | − |
| IRAK1−/− | HEK-293 | − | − | − |
| IRAK1−/− compl. IRAK1 | HEK-293 | +++ | +++ | +++ |
| IRAK4−/− | Human fibroblast | +/− | +++ | +/− |
The indicated cell lines were stimulated with IL-1 for 15 min and analyzed by immunofluorescence for the presence of NEMO or IRAK1 foci and for the nuclear translocation of p65. “IRAK1−/− compl. IRAK1” refers to IRAK1−/− cells transiently reconstituted with IRAK1. “+++” indicates that the event was fully observed (same frequency as for IL-1–treated control cells). “+/−” indicates that the event was observed with a frequency <10% that for control cells. “−” indicates that the event was not observed.