Analysis of complex assemblies by SLS
| Sample | MM exp. | MM calc. | Copy no. |
| kD | kD | ||
| Myo4p-C | 54.9 ± 1.1 | 56.7 | 1 |
| She3p/Myo4p-C | 160.2 ± 3.6 | 153.2 | 2/1 |
| ASH1-E3-51/She2p | 139.2 ± 8.0 | 147.2 | 2/4 |
| ASH1-E3-77-tRNA/She2p | 196.3 ± 5.1 | 211.8 | 2/4 |
| IST2-tRNA/She2p | 176.6 ± 2.6 | 202.1 | 2/4 |
| ASH1-E3-77-tRNA/She2p/She3p/Myo4p-C | 502.8 ± 9.8 | 518.2 | 2/4/4/2 |
| Sample | MM exp. | MM calc. | Copy no. |
| kD | kD | ||
| Myo4p-C | 54.9 ± 1.1 | 56.7 | 1 |
| She3p/Myo4p-C | 160.2 ± 3.6 | 153.2 | 2/1 |
| ASH1-E3-51/She2p | 139.2 ± 8.0 | 147.2 | 2/4 |
| ASH1-E3-77-tRNA/She2p | 196.3 ± 5.1 | 211.8 | 2/4 |
| IST2-tRNA/She2p | 176.6 ± 2.6 | 202.1 | 2/4 |
| ASH1-E3-77-tRNA/She2p/She3p/Myo4p-C | 502.8 ± 9.8 | 518.2 | 2/4/4/2 |
Molecular masses from experimental SLS analysis (MM exp.) compared with calculated molecular masses (MM calc.) for complexes with the indicated copy number of proteins and RNAs. Data represent the means and standard deviation of at least three experiments with varying sample concentrations. Calculated theoretical molecular masses of the monomers are as follows: 56.7 kD (Myo4-C), 48.2 kD (She3p), 28.7 kD (She2p), 16.3 kD (ASH1-E3-51), 48.7 kD (ASH1-E3-77-tRNA), and 43.7 kD (IST2-tRNA).