Table 2. Half-lives of APC/C substrates... | Rockefeller University Press

Table 2.

Half-lives of APC/C substrates

Substrate	Parameter	Control siRNA	siMAD2L2	siMAD2L2:hMAD2L2-myc
Cyclin B1	T_1/2	31.7 ± 0.9	29.6 ± 0.01	44.3 ± 1.5
Cyclin B1	R²	0.947	0.999	0.932
Securin	T_1/2	30 ± 0.9	29.1 ± 0.50	26.8 ± 0.2
Securin	R²	0.938	0.965	0.982
CDC20	T_1/2	50.6 ± 0.6	38.1 ± 0.8	49.2 ± 1.3
CDC20	R²	0.975	0.958	0.950
AURKA	T_1/2	77.0 ± 6.4	46.5 ± 2.1	85.2 ± 3.8
AURKA	R²	0.842	0.913	0.914
UBCH10	T_1/2	106 ± 1.8	91.4 ± 5.8	105 ± 0.9
UBCH10	R²	0.967	0.876	0.983
MAD2L2	T_1/2	51 ± 0.4	–	108 ± 5.3
MAD2L2	R²	0.983	–	0.904

Substrate	Parameter	Control siRNA	siMAD2L2	siMAD2L2:hMAD2L2-myc
Cyclin B1	T_1/2	31.7 ± 0.9	29.6 ± 0.01	44.3 ± 1.5
Cyclin B1	R²	0.947	0.999	0.932
Securin	T_1/2	30 ± 0.9	29.1 ± 0.50	26.8 ± 0.2
Securin	R²	0.938	0.965	0.982
CDC20	T_1/2	50.6 ± 0.6	38.1 ± 0.8	49.2 ± 1.3
CDC20	R²	0.975	0.958	0.950
AURKA	T_1/2	77.0 ± 6.4	46.5 ± 2.1	85.2 ± 3.8
AURKA	R²	0.842	0.913	0.914
UBCH10	T_1/2	106 ± 1.8	91.4 ± 5.8	105 ± 0.9
UBCH10	R²	0.967	0.876	0.983
MAD2L2	T_1/2	51 ± 0.4	–	108 ± 5.3
MAD2L2	R²	0.983	–	0.904

Calculated from fitted exponential decay curves (Fig. 2 E).