Incorporation of [14C]glucose into cell wall polysaccharides during Bgs4 depletion
| Strain | Thiaminea | Incorporation of [14C]glucoseb | |||
| Cell wall | α-Glucanc | β-Glucanc | Galactomannanc | ||
| h | % | % | % | % | |
| Control | 0 (on) | 25.0 ± 2.3 | 22.2 ± 1.9 | 62.7 ± 1.4 | 15.1 ± 0.7 |
| 81X-bgs4+ | 6 (off) | 23.1 ± 0.7 | 33.8 ± 4.0 | 52.4 ± 3.2 | 13.8 ± 0.8 |
| 81X-bgs4+ | 8 (off) | 24.0 ± 1.2 | 40.1 ± 5.1 | 43.2 ± 6.7 | 15.8 ± 1.7 |
| 81X-bgs4+ | 10 (off) | 29.9 ± 0.2 | 49.2 ± 1.0 | 35.6 ± 1.5 | 15.1 ± 0.5 |
| Strain | Thiaminea | Incorporation of [14C]glucoseb | |||
| Cell wall | α-Glucanc | β-Glucanc | Galactomannanc | ||
| h | % | % | % | % | |
| Control | 0 (on) | 25.0 ± 2.3 | 22.2 ± 1.9 | 62.7 ± 1.4 | 15.1 ± 0.7 |
| 81X-bgs4+ | 6 (off) | 23.1 ± 0.7 | 33.8 ± 4.0 | 52.4 ± 3.2 | 13.8 ± 0.8 |
| 81X-bgs4+ | 8 (off) | 24.0 ± 1.2 | 40.1 ± 5.1 | 43.2 ± 6.7 | 15.8 ± 1.7 |
| 81X-bgs4+ | 10 (off) | 29.9 ± 0.2 | 49.2 ± 1.0 | 35.6 ± 1.5 | 15.1 ± 0.5 |
Early log-phase bgs4+-repressed cell cultures were grown in MM + 1.2 M sorbitol for 0, 6, 8, and 10 h in the presence of thiamine (bgs4+-repressed conditions). [14C]glucose was added 4 h before harvesting.
Percentage of incorporation of [14C]glucose = cpm incorporated per fraction × 100/total cpm incorporated. Values are the means and SDs calculated from three independent experiments.
Values are percentages of the corresponding polysaccharide in the cell wall.